肝癌介入治疗后多药耐药机制研究

Mechanism of multidrug resistance in patients with hepatocellular carcinoma after interventional therapy

目的:探讨肝癌介入治疗前后多药耐药相关蛋白(multidrug resistance-associated protein,MRP)基因的表达情况及机制。方法:28例无手术切除指征或拒绝手术治疗的肝癌患者行肝动脉化疗栓塞术治疗,对治疗前后肝癌组织部位行细针穿刺活检,利用实时荧光定量PCR法检测MRP-1、MRP-2、MRP-3、MRP-5基因表达水平。结果:肝动脉化疗栓塞术治疗后MRP-1、MRP-3、MRP-5基因表达水平较治疗前明显增高(P<0.05),治疗前后MRP2基因表达水平差异无统计学意义(P>0.05)。结论:肝癌介入治疗后产生的获得性多药耐药可能与MRP-1、MRP-3、MRP-5基因表达增高有关。

Objective To investigate the expression of genes encoding multidrug resistance-associated protein in hepatocellular carcinoma after interventional therapy and its mechanism.Methods A total of 28 hepatocellular carcinoma patients were treated with transcatheter arterial chemoembolization.Biopsy was performed respectively before and after surgery.The expression levels of multidrug resistance-associated protein 1,2,3 and 5 mRNAs were detected according to the standard curve with real-time fluorescence quantitative PCR technique.Results The expression levels of multidrug resistance-associated protein 1,3 and 5 mRNAs were significantly higher（P0.05）,and the expression of multidrug resistance-associated protein 2 mRNA had no significant difference after therapy（P0.05）.Conclusion The acquired multidrug resistance after transcatheter arterial chemoembolization is correlated with the high expressions of multidrug resistance-associated protein 1,3 and 5 in patients with hepatocellular carcinoma.