摘要
目的:本实验研究了环加氧酶抑制剂对缺血再灌注损伤(ischemia/reperfusion injury,I/R)所致大鼠肾脏组织细胞凋亡、Bcl-2蛋白和超氧化歧化酶(SOD)、丙二醛(MDA)表达的影响。方法:将36只大鼠随机分为3组:对照组,I/R模型组,罗非昔步治疗组,每组12只。I/R大鼠模型采取夹闭双侧肾动脉60min,恢复再灌注24h的方法建立。对照组大鼠除不钳夹双侧肾蒂外,余步骤与手术组同。观察肾组织病理改变,检测肾组织中Bcl-2蛋白、SOD和MDA含量的表达,TUNEL法检测细胞凋亡水平。结果:组织学检查发现对照组肾小管排列整齐,间质无充血水肿,I/R组可见明显损害,肾小管上皮细胞肿胀变性,肾小管扩张,可见管型和坏死脱落细胞,间质充血水肿,炎细胞浸润。治疗组组织损伤较I/R组明显减轻。免疫组化结果半定量分析显示模型组Bcl-2蛋白明显增多,给药组Bcl-2的水平较模型组明显减低(P<0.05)。模型组细胞凋亡明显增多,给药组细胞凋亡水平较模型组明显减低(P<0.05)。模型组肾组织SOD的活性明显降低,MDA含量增高,给药组比模型组有所改善。结论:罗非昔步能抑制I/R肾脏组织Bcl-2蛋白的表达,减轻细胞凋亡,抑制脂质过氧化产物MDA的产生,并提高抗氧化酶SOD的活性,减轻肾脏缺血再灌注损伤。
Objective：To evaluate effects of cyclooxygenase-2 inhibitor on apoptosis of and the expression of Bcl-2 and SOD、MDA in renal tissue after transient ischemia-reperfusion（I/R）injury.Methods：36 rats were divided randomly into 3 groups：normal control groups,12 rats;I/R groups,12 rats;rofecoxib treatment group,12 rats.The rat＇s I/R model was established by clamping bilateral renal arteries for 60 min for ischemia and reperfusing them for 24 hours.The morphological changes was observed.The protein expression of Bcl-2 was measured by immunohistochemistry.The activities of SOD and MDA were examined.The apoptosis rate in renal tissue were measured by TUNEL method.Results：There are tubular normal,no edama in normal control groups.Significant structural changes,including epidermis cell atrophy,tubular dilatation and infiltration of the renal parenchyma with monocytes in I/R groups.These changes are remarkably lighten in rofecoxib treatment group.In comparison with the model group,the levels of Bcl-2 were significantly reduced by rofecoxib treatment（P0.05）.The level of apoptosis were decreased（P0.05）.The activities of SOD were significantly increased.The concentration of MDA were decreased（P0.05）.Conclusion：Rofecoxib may suppress Bcl-2,lighten cell apoptosis.It can decrease the level of MDA and increase the activity of SOD.It has fairly protective effects on I/R of kidney.
出处
《中国中西医结合肾病杂志》
2011年第2期116-118,192,共4页
Chinese Journal of Integrated Traditional and Western Nephrology
