铜绿假单胞菌注射液与5-Fu腹腔化疗对大鼠结肠吻合口愈合影响的对比研究

Comparing study of Pseudomonas aeruginosa injection with 5-Fu intraperitoneal chemotherapy on rat colonic anastomotic healing

目的:比较铜绿假单胞菌(PA)注射液与5-Fu腹腔化疗对大鼠结肠吻合口愈合的影响并探讨其机制。方法:将40只SD大鼠随机均分为4组,即模型对照组、铜绿假单胞菌注射液皮下注射组(PA皮下注射组)、PA注射液皮下注射+术中腹腔喷洒组(皮下注射+腹腔喷洒组)、5-Fu腹腔化疗组(5-Fu组),每组10只,均行结肠部分切除再吻合术。术后7 d检测吻合口的破裂压、病理学形态、胶原纤维含量、转化生长因子(TGF-β)及纤维连接蛋白(FN)表达。结果:PA皮下注射组和PA皮下注射+腹腔喷洒组脏器粘连程度与模型对照组大鼠相近,吻合口破裂压、炎性细胞数量、胶原纤维沉积及TGF-β和FN表达显著高于模型对照组和5-Fu组。结论:PA注射液可提高机体免疫状态,增加吻合口炎性细胞数量,促进结肠吻合口早期愈合,降低吻合口漏的发生,而且不会加重粘连程度。

Objective： To compare the effects of Pseudomonas aeruginosa injection with 5-Fu intraperitoneal chemotherapy on rat colonic anastomotic healing and study its mechanism. Methods： Forty SD rats were randomly divided into 4 groups with 10 rat for each ： control group, Pseudomonas aeruginosa injection group （ subcutaneous injection group）, Pseudomonas aeruginosa injection plus peritoneal spraying group, 5-Fu intraperitoneal chemo- therapygroup group （5-Fu group）. All rats were underwent colon resection and anastomosis. After 7 days, the anas- tomotic bursting pressure, pathology, collagen fiber content, transforming growth factor （TGF-β） and fibronectin protein were detected. Results： The organ adhesion in subcutaneous injection group and Pseudomonas aeruginosa injection plus peritoneal spraying group were similar degree as of control group. Anastomotic bursting pressure, in- flammatory cells, collagen deposition and TGF-β and FN were significantly higher than those of control group and 5- Fu group. Conclusion： Pseudomonas aeruginosa injection can improve the immune status, increase number ofanastomotic inflammatory cells and promote early healing of colonic anastomosis, reduce the incidence of anastomot- ic leakage, decreace scar formation.