反相高效液相色谱法测定人体血浆中克拉维酸钾羟氨苄青霉素的含量

Determination of Clavulanate Potassium and Amoxicllin in Human Plasma by RP-HPLC

目的 :采用反相高效液相色谱法同时测定服用克拉维酸钾羟氨苄青霉素颗粒剂和片剂后人血浆中的克拉维酸和羟氨苄青霉素的浓度。方法 :以ODS柱为分析柱 ,磷酸盐缓冲液 -三乙胺缓冲液 -甲醇 ( 87∶1 3∶2 5) ,用磷酸调 pH至 3作流动相 ,柱温 :3 5℃ ;检测波长 :2 2 2nm。 结果 :本法中羟氨苄青霉素的平均回收率为 99 0 1 %～ 99 75% ,RSD为 1 7%～ 3 2 % ;克拉维酸钾的平均回收率为 97 95%～ 1 0 0 0 % ,RSD为 0 8%～ 2 0 %。 2种药物的日内和日间RSD小于 6 4 % ,血浆中的标准曲线相关良好 ,克拉维酸和羟氨苄青霉素在血浆中的最低检测浓度分别为 0 2 4和 0 2 5μg·mL- 1。结论

Objective:A RP-HPLC method was developed for the determination of clavulanate potassium and amoxicllin in human plasma Methods:Clavulanate potassium and amoxicllin were chromatoghed on ODS column;the mobile phase was phosphatic buffer-triethylamine buffer-methanol(87∶13∶2 5 ) in which pH was adjusted to 3 The column temperature was 35 ℃, and detected at 222 nm Results:The average recovery of clavulanate potassium and amoxicllin was 99 01%～99 75% and 97 95%～100 0% respectively;the minimum detection concentration of clavulanate Potassium and amoxicllin was 0 24 μg·mL -1 and 0 25 μg·mL -1 ,respectively A good linear relationship between the peak area and the components was found at clavulanate potassium was 0 24～14 63 μg·mL -1 , r =0 999 9,and amoxicllin was 0 25～15 14 μg·mL -1 , r =0 999 9 Conclusion:This analytic condition could be used to study the pharmacokinetics and relative bioavailability of clavulanate potassium and amoxicllin pellets and tablets