期刊文献+

高效毛细管电泳柱上富集测定福莫特罗干糖浆 被引量:3

Determination of Formoterol Dry Syrup by Capillary Electrophoresis with Stacking
下载PDF
导出
摘要 目的 :研究了低规格药物福莫特罗干糖浆的含量测定。方法 :采用高效毛细管电泳柱上富集的方法进行测定。电压进样 ,1 0kV ,2 0s,运行电压 2 0kV ,运行缓冲液为 80mmol·L- 1磷酸盐缓冲液 ( pH 3 0 ) ;检测波长为 2 0 0nm。 结果 :福莫特罗浓度在 0 2 5~ 2 0 μg·mL- 1之间线性关系良好 ,r =0 9993 ,n =6;平均回收率为 1 0 0 8% ,RSD =1 5% ,n =5;日内和日间的RSD分别为1 1 % (n =5)和 3 5% (n =3 )。结论 :本法简便 ,准确 ,重现性好 ,样品柱上富集 2 Objective:To study the method for quantitative determination of formoterol dry syrup Methods:A capillary electrophoresis (CE)with head-column field-amplified stacking was selected to determine the content A short water plug was introduced into the inlet of the capillary and then the sample solution was injected with an electrokinetic mode to enhance the sensitivity The running buffer was 80 mmol·L -1 phosphate buffer(pH 3 0),running voltage was 20 kV Chlorcyclizine hydrochloride was selected as internal standard Results:For formoterol the linear range was 0 25~2 0 μg·mL -1 , r =0 999 3 The average recovery was 100 8% with RSD =1 5%( n =5) The limited of quantitation was 30 ng·mL -1 The intra-day precision was 1 1%( n =5)and inter-day precision was 3 5%( n =5) Conclusions:The method was proved to be simple,precise and reproducible The sample was stacked on the column 260-times
作者 陈珏 宋景政
出处 《药物分析杂志》 CAS CSCD 北大核心 1999年第5期325-328,共4页 Chinese Journal of Pharmaceutical Analysis
关键词 毛细管区带电泳 柱上富集 福莫特罗干糖浆 HPLC CE,head-column field amplified stacking,formoterol
  • 相关文献

参考文献4

  • 1Zhang Chaoxuan,Anal Chem,1996年,68卷,2523页
  • 2Chien Ringling,Anal Chem,1992年,64卷,104页
  • 3Chien Ringling,J Chromatogr,1991年,559卷,141页
  • 4福莫特罗干糖浆进口注册标准

同被引文献148

引证文献3

二级引证文献12

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部