糖尿病性勃起功能障碍大鼠的基因表达谱芯片数据分析

Microarray data analysis of genes related with erectile dysfunction in diabetic rats

目的研究糖尿病性勃起功能障碍大鼠基因表达谱芯片数据,应用生物信息学方法挖掘与糖尿病性勃起功能障碍相关的基因,初步探讨其功能及相应分子机制。方法应用GeneSifter在线分析软件对5个正常大鼠阴茎组织和5个糖尿病性勃起功能障碍大鼠阴茎组织基因芯片数据进行分析。结果筛选得到661个差异表达基因,其中280个上调,381个下调。其中kruppel-like factor 5(klf5)基因上调表达4.01倍,ceruloplasmin(cp)基因上调表达5.14倍,collagen,type Ⅺ,alpha1基因下调表达5.84倍,collagen,type Ⅰ,alpha1下调表达5.77倍。661个差异表达基因的功能涉及糖蛋白生物合成、胶原纤维组建、血管发生过程、脂质代谢、细胞增殖等多种重要的生物学过程,以及脂肪酸代谢、神经变性机能紊乱、细胞外基质受体相互作用等信号通路。结论应用生物信息学方法分析糖尿病性勃起功能障碍大鼠基因表达谱发现上调的klf5、cp基因和下调的collagen基因在糖尿病性勃起功能障碍的发病机理中起重要作用,生物信息学方法可为糖尿病性勃起功能障碍发病机制的研究开辟新思路。

Objective To investigate the changes of gene expression profiles associated with erectile dysfunction in diabetic rats.Methods Affymetrix Gene Chip arrays from the Gene Expression Omnibus（GEO） were used to examine the alterations in the gene expression profiles between streptozotocin-induced diabetic rats and littermate controls,and the data were analyzed with GeneSifter microarray analysis software.Results A total of 661 differentially expressed genes were identified,including 280 up-regulated and 381 down-regulated ones.Among the differentially expressed genes,kruppel-like factor 5（klf5） was upregulated by 4.01 folds and ceruloplasmin（cp） by 5.14 folds;collagen,type Ⅺ,alpha1 was down-regulated by 5.84 folds and collagen,type Ⅰ,alpha1 by 5.77 folds.The 661 differentially expressed genes involved such functional processes as glycoprotein biosynthesis,collagen fibril organization,angiogenesis in wound healing,triglyceride metabolism,cell proliferation and other important biological processes,and some pathways also involved such as fatty acid metabolism,neurodegenerative disorders,and ECM-receptor interactions.Conclusions Some genes such as klf5,cp,and collagen play important roles in the pathophysiology of diabetes-induced erectile dysfunction.Bioinformatic approaches offer a new means for identifying candidate genes and pathways relevant to the pathophysiology of diabetes-induced erectile dysfunction,highlighting also the potential complexity of this disorder.