外周血缺氧诱导因子-1αmRNA表达与结直肠癌侵袭转移的关系

Association of peripheral blood hypoxia-inducible factor-1α with metastasis of colorectal cancer

目的探讨外周血缺氧诱导因子-1α(HIF-1α)mRNA表达与结直肠癌侵袭转移的关系。方法采用荧光RT-PCR技术检测我院2010年1月～2010年10月间40例结直肠癌患者及20例健康对照者外周血中HIF-1αmRNA表达情况。结果 40例结直肠癌患者中检测出HIF-1αmRNA表达的17例,检出率为42.5%(17/40),两组比较差异有统计学意义(P<0.01);且HIF-1αmRNA表达与肿瘤分期密切相关(P<0.05)。结论外周血HIF-1αmRNA可作为检测结肠癌患者肿瘤细胞转移的分子标志之一。

Objective To explore the relationship between hypoxia-inducible factor-1α（HIF-1α） mRNA levels in the peripheral blood and the metastasis of colorectal cancer.Methods HIF-1α mRNA in the peripheral blood was detected by RT-PCR in 40 patients with colorectal cancer and 20 healthy subjects.Results Seventeen patients with colorectal cancer showed positivity for HIF-1α mRNA,showing a significantly higher positivity rate（42.5%） than the healthy subjects（P0.05）.The expression of HIF-1α mRNA is closely related to the staging of colorectal cancer（CRC）.Conclusion HIF-1α mRNA may serve as a potential marker in the detection of metastasis of colorectal cancer.