滋阴清嗓汤对咳嗽变异性哮喘发作期患儿IL-4、IL-5水平的影响

Effect of Ziyinqingsang decoction on interleukin-4 and-5 levels in peripheral blood mononuclear cells of children in acute stage of cough variant asthma

目的观察滋阴清嗓汤对咳嗽变异性哮喘(CVA)发作期患儿血清IL-4、IL-5的影响。方法咳嗽变异性哮喘发作期患儿30例,给予中药滋阴清嗓汤治疗2周,用酶联免疫吸附实验(ELISA法)检测治疗前后患儿外周血单个核细胞(PBMC)内IL-4、IL-5水平以及咳嗽、咽痛、喉痒等症状的变化。结果治疗前患儿PBMC IL-4水平为89.69±13.82 ng/l、IL-5为12.17±0.43 ng/ml,治疗后患儿PBMC IL-4水平为72.18±14.89 ng/l、IL-5为5.81±0.31 ng/ml,治疗后血清IL-4、IL-5的水平明显下降,患儿咳嗽、咽痛、喉痒等症状均有改善,差异均有统计学意义(P<0.05)。结论滋阴清嗓汤治疗咳嗽变异性哮喘发作期患儿的作用机制可能与降低血清IL-4、IL-5的水平有关。

Objective To observe the effect of Ziyinqingsang decoction on the levels of interleukin-4（IL-4） and IL-5 in the peripheral blood mononuclear cells（PBMCs） in children with cough variant asthma（CVA） in the acute stage.Methods Thirty children with CVA in the acute stage were given Ziyinqingsang decoction for 2 weeks.The IL-4 and IL-5 levels in the PBMCs were determined using ELISA before and after the treatment,and the changes of the clinical symptoms were observed.Results The levels of IL-4 and IL-5 in the PBMCs was 89.69±13.82 ng/l and 12.17±0.43 ng/ml before the treatment,which were significantly reduced to 72.18±14.89 ng/l and 5.81±0.31 ng/ml after the treatment（P0.05）.The symptoms including coughing,pharyngodynia,and pharyngoitch were all improved obviously（P0.05）.Conclusion Ziyinqingsang decoction ameliorates the symptoms of CVA in the acute stage probably by decreasing the levels of IL-4 and IL-5 in the PBMCs of the children.