单核细胞宫腔灌注联合冻融胚胎移植治疗反复着床失败:附3例报道

Frozen embryo transfer combined with intrauterine administration of autologous peripheral blood mononuclear cells for repeated implantation failure:report of 3 cases

目的探讨自体外周血抽提单核细胞宫腔灌注联合冻融胚胎移植治疗反复着床失败的可能性。方法采用单核细胞经HCG培养48 h后与新鲜抽提单核细胞共同宫腔灌注并联合冻融胚胎移植治疗3例反复着床失败患者,并进行文献复习。结果 3例患者进行上述治疗后均获得妊娠并顺利分娩活产儿,母婴追踪至今未见明显异常。结论外周血单核细胞宫腔灌注联合冻融胚胎移植可试用于反复着床失败患者。

Objective To explore the efficacy of frozen-thawed embryo transfer combined with intrauterine administration of autologous peripheral blood mononuclear cells（PBMCs） in the treatment of repeated implantation failure（RIF）.Methods PBMCs obtained from 3 patients with RIF on the day of follicle rupture（natural cycle） or when the endometrial thickness reached 8 mm（hormone replacement cycle） were cultured in the presence of HCG for 48 h.The cultured PBMCs,along with freshly isolated PBMCs,were administered into the uterine cavity of the patietns.Vitrified cleavage-stage embryos or blastocysts transfer was performed on day 3 or 5,respectively.Results Vitrified embryo or blastocyst transfer resulted in pregnancy and healthy live births in all the 3 patients.Conclusion Frozen-thawed embryo transfer combined with intrauterine administration of autologous PBMCs may be an effective and safe approach to the treatment of RIF and may improve the outcomes of assisted reproduction.