微波组织凝固对荷瘤小鼠全身细胞免疫力的影响

Effect of microwave tissue coagulation on the cellular immunity of the tumor bearing mice

目的 观察微波固化肿瘤后对荷瘤宿主全身细胞免疫力的影响。方法 采用小鼠移植型肝癌H22模型。分别观察微波根治性治疗和姑息性治疗术后1周和2周小鼠细胞免疫力的变化。结果 ①在肿瘤自然生长状态下,小鼠细胞免疫力呈逐渐下降的趋势。②经根治性微波固化治疗后第1周,荷瘤小鼠的细胞免疫力显著高于假手术对照组和切除组(P<0.01),也高于正常对照组。在第2周,虽显著下降(P<0.01),仍显著高于对照组和正常组(P<0.05)。根治性切除治疗后荷瘤小鼠的细胞免疫力无显著变化(P>0.05)。③经姑息性微波固化治疗后第1周,荷瘤小鼠NK活性显著高于假手术对照组(P<0.01),与姑息性切除组和正常对照组差异无显著性(P>0.05),而固化组的巨噬细胞吞噬率显著高于假手术组和切除组(P<0.01)。在姑息性治疗后第2周,固化组的细胞免疫力均高于切除组和假手术组(P<0.01)。切除组与假手术组无显著差别(P>0.05)。结论 根治性微波固化治疗除了能直接杀死肿瘤细胞外,还可诱导出高于正常水平的细胞免疫力。

Objective To determine the effect of microwave tissue coagulation(MTC) on the cellular immunity of the tumor bearing mice.Methods H22 implantable tumor models on mice were applied. This study consists of two parts, which investigate the effect of radical treatment and palliative treatment of MTC on cellular immunity respectively.Results ①The cellular immunity of the tumor bearing mice decreased gradually without intervention.②The cellular immunity of tumor bearing mice in MTC group was much higher than that in excision group and sham surgery group (both P< 0.01 ) one week after the radical treatment, and it is also higher than that in normal control group. At the second week after radical treatment, the cellular immunity of mice in MTC group is still higher than that in excision group and sham surgery group (both P< 0.01 ), though it was lower than that at the first week (P< 0.05 ). ③ At the first week after palliative treatment , the NK activity in MTC group was higher than that in sham surgery group( P< 0.01 ), the macrophage phagocytic rate lymphocyte proliferation rate and IL 2 level in MTC group were higher than those in excision group and sham surgery group ( P< 0.01 ). At the second week after palliative treatment, the cellular immunity was still higher than that in excision group and sham surgery group (P< 0.01 ). There was no significant difference between excision group and sham surgery group.Conclusion Besides killing tumor directly, radical MTC could induce a higher level cellular immunity than normal.