双酚A对雄性大鼠抗氧化能力影响的初步研究

Effect of Bisphenol A on Antioxidant Capacity in Male Rats

目的:探讨双酚A(BPA)对成年大鼠抗氧化能力的影响。方法:将健康成年雄性SD大鼠84只随机分为5个双酚A染毒剂量组(200、50、5、0.5、0.0005 mg/kg)和1个对照组,连续灌胃染毒8周,并监测体重变化,染毒结束后测定血浆超氧化物歧化酶(SOD)和谷胱甘肽-过氧化物酶(GSH-Px)含量;此外,提取肝脏组织RNA,用荧光实时定量PCR测定肝脏硫氧还原蛋白过氧化物酶2(prdx2)mRNA的表达水平。结果:随着染毒时间的延长,50 mg/kg及200 mg/kg剂量组的动物体重增长速度逐渐减慢,自4周开始,与对照组相比有显著差异(P<0.05);染毒结束时,其体重分别为对照组的90.52%和91.61%(p<0.05),而其它低剂量组间则无明显差异;大鼠血浆GSH-Px含量总体上呈下降趋势,且5 mg/kg以上剂量组与对照组相比差别均有统计学意义(p<0.01);染毒组血浆SOD水平虽也低于对照组,但无统计显著性;此外,大鼠肝脏组织prdx2 mRNA水平随双酚A剂量增高呈剂量依赖性降低,与对照组相比,其在最低剂量组(0.0005 mg/kg)即有显著差异(p<0.01)。结论:双酚A可造成大鼠抗氧化酶系统失衡,导致氧化损伤效应,prdx2可能是反映其氧化损伤敏感的生物学标志。

Objective： To investigate the effects of bisphenol A（BPA） on antioxidant capacity of adult Sprague-Dawley rats.Methods： A total of 84 healthy adult male SD rats were divided into 5 different concentration of BPA groups（200,50,5,0.5,0.0005 mg/kg/d） and a control group.The weight of the rats was detected,and the levels of SOD and GSH-Px in the plasma was detected.The mRNA of prdx2 was determined by real-time PCR.Results： The increasing of weight in 50 and 200 mg/kg/d BPA groups got slow day after day from the 4th week;At the end of the experiment,the weight of the two groups reached 90.52 % and 91.61 % of that of control（p0.05）.Plasma GSH-Px had declined in all groups except 0.0005 mg/kg/d compared with that of the control.All BPA groups of plasma SOD were lower than that of control group;The levels of prdx2 mRNA in the rat liver was significant dose-responsed,with BPA increasing,the mRNA decreased.Conclusions： BPA caused unbalance of antioxidant enzyme system of adult male SD rats,and led to oxidative damage.Prdx2 may be the sensitive biomarker of oxidative damage.