期刊文献+

近红外标记的维甲酸类造影剂用于骨肉瘤成像的研究

Application of NIR-Retinoid Imaging Agent in Detection of Human Osteosarcoma
原文传递
导出
摘要 目的:开发一种具有"找寻、治疗、可视"功能的生物造影剂。方法:采用化学合成的方法得到近红外标记的维甲酸类造影剂,并进行骨肉瘤细胞的体外结合试验;皮下接种裸鼠,构建骨肉瘤的异种移植模型,持续10d对裸鼠进行体内光学成像,观察药物在体内的重新分布,并最终用免疫组织化学法对成像结果进行验证。结果:体外细胞结合试验证明,合成的维甲酸造影剂可以很好的与人的骨肉瘤细胞相结合,进而内化。近红外光学成像表明,该造影剂可用于骨肉瘤的早期和晚期诊断。全身成像显示了在肿瘤和肝脏的高信号强度。正电子发射断层显像(PET)显示肿瘤部位具有较高水平的18F-FDG代谢。剂量增加反应和毒性试验表明,高剂量的维甲酸造影剂必然与其全身毒性息息相关。免疫组化染色显示,发光组织中肿瘤细胞呈阳性。结论:合成的近红外标记的维甲酸造影剂可以用于检测人类骨肿瘤的异种移植,实现个体化分子诊疗的同时减少全身毒性。 Objective: To develop a kind of biological contrast agent with the features of "finding,treatment,visual".Methods: Retinoid imaging agents are synthesized and labeled with near-infrared dye for in vitro cell binding studies on human osteosarcoma cell lines.Xenograft model was establish by subcutaneous injection(s.c.);In vivo optical imaging studies were carried out for days after injection,drug redistribution in the body was detected.Tumor cells in the luminous tissue wre detected by immunochemistry.Results: Those agents bound to the human osteosarcoma cells,and internalized,which can be used to detect osteosarcoma at early stages of the metastatic disease.Whole body images showed the high signal intensity in tumor and liver.PET showed that there was high level of 18F-FDG in tumors.The high dose of retinoid agent was significantly associated with systemic toxcity.Immunochemistry demonstrated tumor cells were positive in the luminous tissues.Conclusion: Synthetic NIR-labeled retinoid agents can be used to detect human cancer xenografts as the agent is internalized by cancer cells,and it can reduce systemic toxicity.
出处 《现代生物医学进展》 CAS 2011年第8期1424-1427,共4页 Progress in Modern Biomedicine
基金 国家自然科学基金面上项目(30873027 30973409)
关键词 分子成像 细胞摄取 光学成像 肿瘤 近红外 Molecular imaging Cellular uptake Optical imaging Cancer Near-infrared
  • 相关文献

参考文献16

  • 1Hansen LA, Sigman CC, Andreola F, et al. Retinoids in chemopreven - tion and differentiation therapy [J]. Carcinogenesis, 2000,21 (7): 1271-1279.
  • 2Mongan NP, Gudas LJ. Diverse actions of retinoid receptors in cancer prevention and treatment. Differentiation[J]. 2007, 75(9):853-870.
  • 3丁桂聪,毛天球,黄思东,束煌.维甲酸对口腔黏膜上皮细胞增殖的影响[J].广东牙病防治,2008,16(7):303-305. 被引量:2
  • 4陈万涛,蔡殿才,李宁毅,徐骎,蒋欣泉,王明国,何荣根.RARβ基因联合维甲酸体内抗口腔鳞癌作用机制研究[J].口腔颌面外科杂志,2005,15(1):9-12. 被引量:5
  • 5Braams JW, Pruim J, Freling NJ, et al. Detection of lymph node metastases of squamous-cell, cancer of the head and neck with FDG-PET and MRI[J]. J Nucl Med, 1995,36(2):211-216.
  • 6Duhaylongsod FG, Lowe VJ, Patz EF, Jr., et ai. Detection of primary and recurrent lung cancer by means of F-18 fluorodeoxyglucose positron emission tomography (FDG PET)[J]. J Thorac Cardiovasc Surg, 1995, 110(1):130-140.
  • 7lnoue T, Kim EE, Komaki R, et al. Detecting recurrent or residual lung cancer with FDG-PET[J]. J Nuel Med, 1995,36(5):788-793.
  • 8Escalona S, Blasco JA, Reza MM, et al. A systematic review of FDG-PET in breast cancer[J]. Med Oncol, 2010,27(1): 114-129.
  • 9Dassen AE, Lips DJ, Hoekstra C J, et al. FDG-PET has no definite role in preoperative imaging in gastric cancer [J]. Eur J Surg Oncol, 2009,35(5):449-455.
  • 10Heusner TA, Hahn S, Hamami ME, et al. Incidental head and neck (18)F-FDG uptake on PET/CT without corresponding morphological lesion: early predictor of cancer development? [J]. Eur J Nucl Med Mol Imaging, 2009, 36(9):1397-1407.

二级参考文献19

  • 1邵志敏,沈镇宙.维甲酸核受体-β抑制乳腺癌细胞的生长[J].中华肿瘤杂志,1996,18(5):324-327. 被引量:4
  • 2Lotan R. Supression of sqamous cell carcinoma growth and differentiation by retinoids[J]. Cancer Res, 1994, 54:1987-1990.
  • 3Malcolm AS, David RP, Bruce DC, et al. Retinoids in cancer therapy[J]. J Clin Oncol, 1992,10(5):839-864.
  • 4CHEN Wan-tao, HE Rong-gen, ZHANG Zhi-yuan, et al.Enhanced efficacy of the combination of All-Trans-Retinoic Acid and γ-Interferon in inhibiting proliferation of Tca8113 cells[J]. T Chin J Dent Res, 1999, 2(1): 54-60.
  • 5XU Xiao-chun, Jae YR, Jin SL, et al. Differential expression of nuclear retinoid receptors in normal, premalignant,and malignant head and neck tissues [J]. Cancer Res,1994, 54:3580-3587.
  • 6Nadine D, Giorgio S, Carol J, et al. Loss of retinoic acid receptors in mouse skin and skin tumors is associated with activation of the rasHa oncogene and high risk for premalignant progression[J]. Cancer Res, 1996, 56:4942-4949.
  • 7Scott ML, Steven EB, Waua KH. Cancer chemopevention[J]. J Clin Oncol, 1994,12(4): 851-873.
  • 8VICANOVA J, BOELSMA E, MOMMOL/LAAS M A, et al. Normalization of epidermal calcium distribution profile in reconstructed human epidermis is related to improvement of terminal differentiation and stratum corueum barrier formation. J Invest Dermatol, 1998,111( 1 ) :97-106.
  • 9KAUTSKY M B, FLECKMAN P, DALE B A. Retinoic acid regulates oral epithelial differentiation by two mechanisms. J Invest Dermatol, 1995,104(4) :546-553.
  • 10POUMAY Y, PITFELKOW M R. Cell density and culture factors regulate keratinocyte commol/Lunication to differentiation and expression of superbasal k1/k10 keratins. J Invest Dermatol, 1995, 104(2) :271-276.

共引文献5

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部