TNRC9基因rs12443621A/G多态性与中国女性乳腺癌易感性及临床病理关系的研究(英文)

Implication of the TNRC9 rs12443621A/G Polymorphisms in BreastCancer in Chinese Women

目的:研究TNRC9/LOC643714基因rs12443621A/G多态性与乳腺癌易感性及临床病理之间的关系。方法:DNA试剂盒提取321例乳腺癌患者和340例正常女性静脉血全基因组DNA,PCR扩增目的基因片段,提取扩增样本进行DNA测序检测分析rs12443621多态性。应用SPSS17.0软件对实验结果进行统计学分析。结果:应用SPSS17.0软件对TNRC9/LOC643714基因rs12443621A/G多态性AA、AG、GG进行卡方检验分析,结果显示三种基因型分布在病例组及对照组中无统计学意义(X2=1.43,P>0.05),与乳腺癌易感性无关,与乳腺癌病理分型、ER、PR、HER-2状态以及淋巴结是否转移无相关性(X2=2.90,P>0.05;X2=2.25,P>0.05;X2=1.671,P>0.05;X2=1.34,P>0.05;X2=3.24,P>0.05)。结论:TNRC9基因rs12443621A/G多态性与乳腺癌易感性及临床病理特征无关,不能作为独立的基因标志物对乳腺癌进行早期检测和诊断。

Objective： To investigate the relationship between the rs12443621 polymorphisms of TNRC9/ LOC643714 and breast cancer risk and clinico-pathological characteristics in Chinese women.Methods： Genomic DNA was extracted from peripheral blood.The Single Nucleotide Polymorphisms of the TNRC9/LOC643714 rs12443621,from breast cancer of 321 cases and 340 controls,were detected by polymerase chain reaction（PCR） and direct DNA sequencing.Results： The genotypes of rs12443621 can not increase the risk for breast cancer（X2=1.43,P0.05）.There was no relationship between the genotypes and pathological category,lymph node metastases,ER status or PR status or Her-2 status（X2=2.90,P0.05;X2=2.25,P0.05;X2=1.671,P0.05;X2=1.34,P0.05;X2=3.24,P0.05）.Conclusion： There was no relationship between three genotypes of rs12443621A/G and individual susceptibility or clinic pathological characteristics of breast cancer.