IL-4和IL-12在宫颈癌中的表达及对紫杉醇过敏的影响(英文)

Significance of IL-4 and IL-12 in Cervical Cancer and Relation to Taxol Allergy

目的:研究IL-4,IL-12在宫颈癌组织中的表达,探讨其对宫颈癌发生及术后对紫杉醇过敏的影响。方法:应用半定量逆反应-聚合酶链反应(RT-PCR)技术检测IL-4mRNA,IL-12p35以及IL-12p40 mRNA在正常宫颈组和宫颈癌组中的表达,并分析两者之间的相关性以对紫杉醇过敏的影响。结果:1.宫颈癌组中IL-4mRNA表达水平高于正常宫颈组,而IL-12p35和IL-12p40mRNA表达低于正常宫颈组,差异有统计学意义(P<0.05);2.在术后给予紫杉醇治疗的宫颈癌患者中,过敏组中IL-4mRNA的表达高于不过敏组;IL-12p35和IL-12p40mRNA则低于后者,差异有统计学意义(P<0.05)。结论:体内IL-12降低和(或)IL-4升高可促进宫颈癌的发生发展增加紫杉醇过敏的发生率。

Objective： To investigate the expression of IL-4 and IL-12 in the cervical cancer and to investigate the relationships between the cervical carcinoma and Taxol allergy.Methods： Total RNA was extracted from paraffin-embedded tissues which were from 35 cases of cervical cancer and 15 cases of normal cervix.The results were analyzed by the quantitation access to densitometric scan of agarose gel electrophoresis photos.Results： 1.The expression of IL-4 in the cervical carcinoma was higher than that in normal cervical tissues while the expressions of IL-12p35 and IL-12p40 had the opposite tendency.2.The expression of IL-4mRNA in allergic groups was higher than that in control groups during Taxol therapy with cervical cancer.IL-12p35mRNA and IL-12p40mRNA decreased.Conclusions： The increasing of IL-4 and（or） the dropping of IL-12 might promote cervical diseases and increase the risk of taxol allergy after the operation.