基质金属蛋白酶-9表达与胰腺癌生物学行为及预后的相关性研究

Correlation between MMP-9 Expression and Biological Behavior and Prognosis of Pancreatic Cancer

目的:探讨基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)的表达与胰腺癌生物学特性及对患者预后的影响。方法:采用免疫组织化学法检测63例原发性胰腺癌及11例癌旁正常组织中MMP-9的表达.比较MMP-9的表达与胰腺癌患者生物学特性及预后的关系。结果:MMP-9阳性表达率为68.3%(43/63),而11例癌旁正常组织无MMP-9表达。MMP-9的表达与肿瘤大小(P=0.014)、分化程度(P=0.039)、TNM分期(P=0.001)、远处转移(P=0.011)及淋巴结转移(P=0.009)显著相关。MMP-9表达阳性者生存时间明显低于阴性表达者(Log-rank检验,x2=32.70,P=0.000)。Cox回归分析表明:分化程度(P=0.000)、TNM分期(P=0.006)、淋巴结转移(P=0.035)及MMP-9过表达(P=0.000)是胰腺癌预后的独立影响因素。结论:MMP-9过表达在胰腺癌的浸润转移中发挥重要作用,过表达提示预后不良.对MMP-9的检测可有效的评估胰腺癌患者的预后。

Objective： To evaluate the relationship between expression of matrix metalloproteinase-9（MMP-9） and its biological characteristic,pognosis in pancreatic cancer patients.Methods：The expression of MMP-9 in 63 patients with pancreatic cancer and in 11 patients with the pancreatic tissue of noncarcinoma was examined by immunohistochemical methods.The expression of MMP-9 was compared with biological characteristics and prognosis of pancreatic cancer.Results： The positive expression rate of MMP-9 was 68.3%（43/63）,and there was no expression of MMP-9 in adjacent normal pancreatic tissues.The expressions of MMP-9 were significantly correlated with tumor size（P=0.014）,differentiation（P=0.039）,TNM stages（P=0.001）,distant metastasis（P=0.011） and lymph node metastasis（P=0.009）.And the survival time of pancreaic cancer patients with the positive expression of MMP-9 was shorter than the negative expression（Log-rank test,x2=32.70,P=0.000）.The Cox regression showed that differentiation（P=0.000）,TNM stages（P=0.006）,lymph node metastasis（P=0.035）,MMP-9 overexpression（P=0.000） were related with prognosis of pancreatic cancer patients.Conclusion： The overexpressions of MMP-9 proteins play important roles in proliferation and progression in pancreatic cancer,the overexpression of MMP-9 suggests the worse prognosis in pancreatic cancer.The examination of MMP-9 is useful in evaluating the prognosis of pancreatic cancer patients.