小花草玉梅化学成分研究

Triterpenoid Saponins from Anemone Rivularis var.Flore-minore

目的:研究银莲花属植物小花草玉梅的化学成分。方法:采用硅胶柱色谱,凝胶柱色谱,反相柱色谱并结合制备高效液相色谱等技术分离纯化单体化合物,并根据理化性质及光谱数据鉴定结构。结果:分离并鉴定了4个化合物,分别是常春藤皂苷元-28-O-β-D-吡喃葡萄糖酯苷(1)、3-O-β-D-吡喃葡萄糖-(1→2)-α-L-吡喃阿拉伯糖-齐墩果酸皂苷元-28-O-α-L-吡喃鼠李糖-(1→4)-β-D-吡喃葡萄糖-(1→6)-β-D-吡喃葡萄糖酯苷(2)、3-O-β-D-吡喃葡萄糖-(1→2)-α-L-吡喃阿拉伯糖-常春藤皂苷元-28-O-α-L-吡喃鼠李糖-(1→4)-β-D-吡喃葡萄糖-(1→6)-β-D-吡喃葡萄糖酯苷(3)和3-O-β-D-吡喃核糖-(1→3)-α-L-吡喃鼠李糖-(1→2)-α-L-吡喃阿拉伯糖-常春藤皂苷元-28-O-α-L-吡喃鼠李糖-(1→4)-β-D-吡喃葡萄糖-(1→6)-β-D-吡喃葡萄糖酯苷(4)。结论:化合物1为首次从银莲花属植物中分离得到,2-4为首次从小花草玉梅中分离得到。

Objective： To investigate the chemical constituents of Anemone rivularis var.flore-minore Maxim.Methods： The chemical constituents of the Anemone rivularis var were isolated and purified by various chromatographic methods,and then the structures were identified by physicochemical properties and spectral analysis.Results： Four compounds were identified as hederagenin-28-O-β-D-glucopyranosyl ester（1）,3-O-β-D-glucopyranosyl-（1→2）-α-L-arabinopyranosyl-oleanolicacid-28-O-α-L-rhamnopyranosyl-（1→4）-β-D-glucopyranosyl-（1→6）-β-D-glucopyranosyl ester（2）,3-O-β-D-glucopyranosyl-（1→2）-α-L-arabinopyranosyl-hederagenin-28-O-α-L-rhamnopyranosyl-（1→4）-β-D-glucopyranosyl-（1→6）-β-D-glucopyranosyl ester（3）,3-O-β-D-ribopyranosyl-（1→3）-α-L-rhamnopyranosyl-（1→2）-α-L-arabinopyranosyl-hederagenin-28-O-α-L-rhamnopyran-osyl-（1→4）-β-D-glucopyranosyl-（1→6）-β-D-glucopyranosyl ester（4）,on the basis of NMR spectra.Conclusion： compound 1 was isolated from Anemone for the first time and 2-4 were isolated from Anemone rivularis var.flore-minore Maxim.for the first time.