响应面分析法优化龙眼核中多酚物质提取工艺

Optimization of Extraction Process for Polysaccharides from Longan Seeds by Response Surface Methodology

目的:利用响应面法对龙眼核中多酚物质的提取工艺进行优化。方法:在单因素试验基础上选取试验因素与水平,根据Box-Behnken Design(BBD)试验设计原理采用三因素三水平的响应面分析法,依据回归分析确定各工艺条件的影响因素,以龙眼核多酚物质含量为响应值作响应面和等高线图。结果:在分析各个因素的显著性和交互作用后,得出龙眼核多酚物质浸提的最佳工艺条件为乙醇体积分数70%、浸提温度77.4℃、浸提时间4h、料水比1:20(g/mL)、浸提2次,以焦性没食子酸为标准品,龙眼核多酚物质一次提取含量可达21.7079mg/g。结论:曲面回归方程与实验结果拟合性好,此模型合理可靠,可用于实际预测。

Objective： To optimize conditions for the extraction of polysaccharides from longan seeds.Methods： Based on single factor experiments,the optimal conditions for polysaccharide extraction were explored by Box-Behnken design（BBD） in combination with response surface methodology（RSM）.Results： The optimal extraction conditions of polysaccharides were ethanol concentration of 70%（V/V）,extraction temperature of 77.4 ℃,extraction time of 4 h,solid-to-liquid ratio of 1：20（g/mL） and extraction number of 2.Temperature was the most important affecting factor of polysaccharide extraction,followed by extraction time and ethanol concentration.Under such extraction conditions,the experimental yield of polysaccharides obtained after the first extraction was up to 21.7079 mg/g,which was close to the model-predicted value.Conclusion： The established regression equation for polysaccharide yield has an excellent goodness of fit.Therefore it is credible and can be used for practical prediction