胚蛋注射谷氨酰胺对肉仔鸡早期免疫器官的影响

Effects of in Ovo Feeding Glutamine on Early Immune Organs in Broiler

选用已孵化18 d的肉仔鸡活胚蛋240枚,随机分成4个处理组,每个处理6个重复,每个重复10枚蛋。处理组Ⅰ注射1 mL 7.5 g/L生理盐水作为对照,处理组Ⅱ、Ⅲ、Ⅳ分别向鸡胚羊膜腔内注射质量浓度为51、0 mL/L和15 mL/L的谷氨酰胺营养液1 mL。结果表明,胚蛋注射谷氨酰胺对3日龄肉仔鸡的胸腺、脾脏和法氏囊重量及其器官指数没有影响(P>0.05)。7日龄肉仔鸡胸腺重量因胚蛋注射谷氨酰胺而有提高的趋势(P>0.05),10 mL/L和15 mL/L注射组胸腺指数分别较对照组提高了22.67%和19.56%(P<0.05);5 mL/L注射组与对照组相比脾脏重量提高25.00%(P<0.05),脾脏指数提高24.53%(P<0.05),10 mL/L注射组与对照组相比脾脏重量提高31.25%(P<0.05),脾脏指数提高32.08%(P<0.05);各试验组法氏囊重量及指数较对照组差异不明显(P>0.05)。

In this study,240 hatching broiler eggs at 18 d incubation were randomly divided into 4 treatments groups with 6 replicates of 10 eggs each.Eggs in group one were injected with 1 mL 7.5 g/L physiological saline into amniotic cavity as control,and those in group two,three and four were injected into amniotic cavity with 1 mL 5 mL/L,10 mL/L and 15 mL/L glutamine solution,respectively.The results showed that the weights and organ indexes of thymus,spleen and bursa of fabricius in 7 d broilers in ovo fed with glutamine had no statistical differences compared with those in the control group（P0.05）.The thymus weights of 7 d broilers showed increased trend（P0.05）.And the thymus indexes of broilers in ovo treated with 10 mL/L and 15 mL/L glutamine increased 22.67% and 19.56%（both at P0.05） respectively.The spleen weight of broilers in ovo treated with 5 mL/L glutamine increased 25.00% compared with that of the control group（P0.05）,and the spleen indexes increased by 24.53%（P0.05）.The spleen weight and spleen index of broilers in ovo treated with 10 mL/L glutamine increased by 31.25% and 32.08%（P0.05） respectively.However,the weight and organ index of bursa of fabricius in all test groups showed no statistical differences compared with the control group（P0.05）.