人工诱导龙血竭与野生龙血竭化学成分的比较研究

The comparative study on the chemical constituents of artificial induced and wild Dragon's Blood

对人工诱导龙血竭与野生龙血竭的化学成分进行比较研究,为人工诱导龙血竭是否能替代其野生龙血竭提供依据,解决龙血竭资源极其紧张的问题.采用HPLC法.选用Waters Nova-Pak C18色谱柱(Ф3.9 mm×150 mm,5μm),检测波278 nm,柱温40℃,进样量10μL,以乙腈-0.4%磷酸(体积比30∶70)为流动相;流速1.0 mL/min.结果:龙血竭和人工诱导龙血竭均具有相对保留时间14.2,19.9 min的2个共有峰,且HPLC峰形相似度较高.

To solve the problem of Dragon＇s Blood resource exhaustion,the chemical constituents of artificial induced and wild Dragon＇s Blood were compared,and the evidence whether artificial induced Dragon＇s Blood can replace wild Dragon＇s Blood was provied.Using HPLC,Waters Nova-Pak C18 column.detection wavelength 278nm,column temperature 40℃,acetonitrile-0.4% phosphate（V/V=30∶70） as mobile phase,flow rate 1mL/min and injection volume 10μL.Both artificial induced and wild Dragon＇s Blood have two common peaks,relative retention rime 4.2,19.9min,and the similarity degree of HPLC chromatographic peak shape was high.Therefore,the chemical constituents of artificial induced and wild Dragon＇s Blood were similar.Artificial induced Dragon＇s Blood can replace wild Dragon＇s Blood,but pharmacological experiments were still needed for further study of their differences.