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Rho激酶抑制剂诱导PC12和PC12Adh细胞突起生长的差异比较

Differences of neurite outgrowth induced by rho kinase inhibitors between in PC12 cell line and PC12 Adh cell line
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摘要 PC12细胞是研究神经分化最常用的细胞之一.在rho激酶(ROCK)抑制剂的作用下,PC12细胞能够长出神经样突起.最近,美国菌种保存中心(ATCC)同时提供PC12细胞和PC12 Adh细胞.研究的主要目的是观察ROCK抑制剂诱导这2种细胞长突起是否存在差异.PC12细胞和PC12Adh细胞按照ATCC方法进行培养,用神经生长因子(NGF,1 000 ng/mL)或ROCK抑制剂(33μmol/L Y27632,33μmol/L法舒地尔)处理细胞1~4 d.结果发现NGF能够诱导这2种细胞生长突起,而ROCK抑制剂只诱导PC12Adh细胞长突起,对PC12细胞不明显.因此,ROCK抑制剂诱导这2种细胞突起生长存在明显差异,PC12Adh细胞更适合用于ROCK抑制剂的神经诱导分化实验. PC12 cell line is one of the basic tool cell lines used in neural differentiation research.The cell line is able to grow neurite by exposure to rho kinase(ROCK) inhibitors.Recently,American Type Culture Collection(ATCC) provided PC12 cell line and PC12 Adh cell line.The main aim of the study was to clarify the differences of the two cell lines in neurite outgrowth induced by ROCK inhibitors.PC12 cell line and PC12 Adh cell line(from ATCC) were treated with nerve growth factor(NGF,1000ng/mL) or ROCK inhibitors(Y27632 of 33 μmol/L and fasudil of 33 μmol/L) for 1 —4 days.NGF was able to promote neurite outgrowth both in PC12 cell line and PC12 Adh cell line,while ROCK inhibitors was able to do so only in PC12 Adh cell line.These results suggested that PC12 Adh cell line is more suitable for ROCK inhibitors study in neural differentiation.
出处 《云南大学学报(自然科学版)》 CAS CSCD 北大核心 2011年第3期370-372,F0002,共4页 Journal of Yunnan University(Natural Sciences Edition)
基金 国家自然科学基金资助项目(81060109) 云南省科技计划面向资助项目(2008CD150)
关键词 RHO激酶抑制剂 突起生长 PC12Adh细胞 PC12细胞 神经生长因子 rho kinase inhibitor neurite outgrowth PC12 Adh cell line PC12 cell line nerve growth factor
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  • 1GREENE L A, TISCHLER A S. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor [ J ], Proc Natl Acad Sci U S A,1976,73(7) :2424-2 428.
  • 2裴新,陆地,周宇冠,陈穗云.雌激素对H_2O_2诱导PC12细胞氧化应激的保护作用研究[J].云南大学学报(自然科学版),2010,32(5):608-612. 被引量:6
  • 3VAUDRY D, STORK P J, LAZAROVICI P, et al. Signaling pathways for PC12 cell differentiation: making the right connections [ J ]. Science, 2002, 296 ( 5 573 ) : 1 648-1 649.
  • 4FUJITA A, HATTORI Y, TAKEUCHI T, et al. NGF induces neurite outgrowth via a decrease in phosphoryla- tion of myosin light chain in PC12 cells [ J ]. Neuroreport ,2001,12( 16 ) :3 599-3 602.
  • 5GOPALAKRISHNAN S M, TEUSCH N, IMHOF C, et al. Role of rho kinase pathway in chondroitin sulfate proteoglycan - mediated inhibition of neurite outgrowth in PC12 cells [ J ]. J Neurosci Res, 2008,86 (10) : 2 214-2 226.
  • 6ATCC. CRL - 1721 product details [ EB/OL]. [ 2010 - 11 -26]. http://www, atcc. org/ATCCAdvancedCata- logSearch/ProductDetails/tabid/452/Defauh, aspx? ATCCNum = CRL - 1721 &Template = cellBiology.
  • 7ATCC. CRL - 1721.1 product details [ EB/OL]. [ 2010 - 11 -26]. http ://www. atcc. org,/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default, aspx? ATCCNum = CRL - 1721.1 &Template = cellBiology.

二级参考文献18

  • 1SHI Q, GIBsoN G E. Oxidative stress and transcriptional regulation in Alzheimer disease [ J ]. Alzheimer Dis Assoc Disord,2007,21:276-291.
  • 2ENNIO E,DOMENICO R,Vincenzo Di Matteo,et al. A review of specific dietary antioxidants and the effects on biochemical mechanisms related to neurodegenerative processes [ J ]. Neurobiology of Aging, 2002,23 : 719- 735.
  • 3COYLE J T, PUTTFARCKEN P. Oxidative stress, glutamate, and neurodegenerative disorders [ J ]. Science, 1993,262(5 134) :689-695.
  • 4DIZDAROGLU M,JARUGA P, BIRINCIOGLU M,et al. Free radical- induced damage to DNA: mechanisms and measurement [ J ]. Free Rad Biol Med, 2002,32 : 1 102-1 115.
  • 5SIMPKINS J W, YANG S H, LIU R, et al. Estrogen - like compounds for ischemic neuroprotection [ J ]. Stroke ,2004,35:2 648-2 651.
  • 6SIMPKINS J W, WANG J, WANG X, et al. Mitochondria play a central role in estrogen - induced neuroprotection [ J ]. CNS & Neurol Disorders - Drug Targets, 2005,4 : 69-83.
  • 7CHU C T,LEVINTHAL D J, KULICH S M, et al. Oxidative neuronal injury. The dark side of ERK1/2[ J]. Eur J Biochem ,2004,271:2 060-2 066.
  • 8MARIANI E, POLIDORI M C, CHERUBINI A, et al. Oxidative stress in brain aging, neurodegenerative and vascular diseases : an overview [ J ]. J Chromatogr B : Analyt Technol Biomed Life Sci,2005,827( 1 ) :65-75.
  • 9EBERLING J L, WU C, Tong - Tumbeaugh R. Estrogen -and tam oxifen -associated effects on brain structure and function[ J]. Neuro in age,2004,21 (1) :364-371.
  • 10WOOTEN G F, CURRIE L J, BOVBJERG V E, et al. Are men at greater risk for Parkinson' s disease than women [ J ]. Neurol Neurosurg Psychiatry, 2004, 75 (4) :637-639.

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