胃癌组织中血管生成素-2基因的表达和微血管密度研究

Study on expression of Angiopoietin-2 and microvessel density in patients with gastric carcinoma

目的探讨血管生成素-2(Ang-2)基因在胃癌组织中的表达、微血管密度(MVD)与胃癌生物学行为之间的关系。方法采用免疫组化SP方法,检测64例胃癌组织、22例癌旁正常组织、20例胃良性疾病组织中血管生成素(Ang-2),以CD34作为血管内皮细胞标记物,对CD34阳性血管进行MVD计数。结果 Ang-2在胃癌组织中的阳性表达率为68.75%,在癌旁正常组织和胃良性病变组织中的阳性表达率分别为22.27%、40.00%,差异有统计学意义(P<0.01);Ang-2表达与胃癌浸润深度、淋巴结转移、远处转移、临床分期呈正相关(P<0.05),Ang-2阴性表达者5年总生存期明显高于Ang-2阳性表达组(分别为50.00%及18.75%,P<0.05);胃癌组织、癌旁组织和胃良性病变组织中MVD分别为33.69±11.60、10.65±3.00和17.20±1.98,3者比较差异有统计学意义(P<0.01);MVD与浸润深度、淋巴结转移、远处转移及临床分期呈正相关(P<0.01),而与肿瘤大小、分化程度、有无脉管侵犯无相关(P>0.05);MVD≤33的胃癌患者总生存期较MVD>33患者明显延长(分别为45.45%及16.67%),差异有统计学意义﹙P<0.05)。经相关分析,Ang-2阳性表达率与MVD呈正相关(r=0.618,P<0.01)。结论 Ang-2的表达和MVD与胃癌的生长、浸润、转移及预后有一定的相关性。

Objective To investigate the expression of Angiopoietin-2（Ang-2） and colletlation of microvessel density（MVD） in patients with gastric carcinoma and discuss their significance in carcinogenesis progression,invasion and metastasis in patients with gastric cancer,as well as their relationship with biological behavior of gastric cancer.Methods The expressions of Ang-2,MVD were studied by immunohistochemistry in 64 gastric carcinoma specimens and 22 normal tissues,20 patients with benign gastric lesion.The MVD was assayed by CD34 positive blood vessels counting.Results The positive rates of Ang-2 were respectively 68.75%,22.27% and 40.00% in gastric carcinoma and normal tissues,patients with benign gastric lesion.Expressions of Ang-2 proteins were significantly（all P〈0.01）.The positive expression rates of Ang-2 were significantly correlated with depth of invasion,lymph node metastasis,distant metastasis and clinical stage in gastrointestinal carcinoma patients（P〈0.05）;The five-year overall survival was lower in patients with tumors positive for Ang-2 than in those with tumors negative for Ang-2（50.00% vs.18.75%）.The MVD score in gastric carcinoma was significantly higher than that in normal tissues,patients with benign gastric lesion（P〈0.01）,（33.69±11.60 vs.10.65±3.00 and 17.20±1.98）.The MVD in gastric cancer were significantly associated with depth of invasion,lymph node metastasis,distant metastasis and clinical stage（P〈0.05）,but there were not correlated with differentiation,tumor size and vascular of invasion（P〉0.05）.The five-year overall survival was lower in patients with tumors having high MVD score than in patients with tumors having low MVD score（45.45% vs.16.67%,P 0.05）.In gastric carcinoma,expression had a positive correlation with Ang-2 and MVD（r=0.618,P〈0.01）.Conclusion Ang-2 and MVD are related to the growth,infiltration,metastasis and prognosis of gastric carcinoma.