摘要
目的：建立高效液相色谱法测定云南松松针中莽草酸的含量方法。方法：色谱柱为Agilent Eclipse XDB-C18（4.6mm×150mm,5μm）,流动相为甲醇-1%磷酸水溶液（3：97）,流速0.8m.lm in-1,检测波长214nm,柱温25℃。结果：莽草酸在0.0312-0.4368ug范围内与峰面积呈良好的线性关系（r=0.9999）,平均加样回收率为99.97%,RSD为1.06%（n=6）。结论：此方法准确、简便,适用于云南松松针中莽草酸的定量分析。
Objective： To establish an analytical method of shikimie acid in pine needle of pinus yunnanensis by high performance liquid chromatographic method.Methods：Using Agilent Eclipse XDB-C18 column（4.6mm×150mm,5μm）,mobile phase wasmethanol-1%phosphoric acid solution（3：97）,the flow rate was 0.8ml·min-1,the detection wavelength was 214 nm,column temperature was 25℃.Results： The result indicated good linearity over the range of 0.0312～0.4368ug with coefficients（r） of 0.9999,the average recorvery was 99.97%,RSD was 1.06%（n=6）.Conclusion： The method is convenient and simple for the determination of shikimie acid in pine needle of pinus yunnanensis.
出处
《中国民族民间医药》
2011年第9期38-39,共2页
Chinese Journal of Ethnomedicine and Ethnopharmacy
基金
大理学院大学生科研基金项目
关键词
云南松松针
莽草酸
高效液相色谱法
pine needle of pinus yunnanensis
shikimie acid
high performance liquid chromatographic
