不同硒营养和运动对小鼠免疫和抗氧化功能的影响

THE INFLUENCE OF SELENIUM AND EXERCISE ON IMMUNE AND ANTIOXIDATIVE FUNCTIONS IN MICE

目的： 研究小鼠长期训练时不同硒营养对免疫、抗氧化功能的影响。方法： 四周龄 Ｃ５７ Ｂ Ｌ／６ Ｊ雄性小鼠按饲料硒水平分为低硒、正常硒、补硒组。每组分为两个亚组：训练组和安静组。各组喂以相同饲料，但给适硒，补硒组添加硒酸钠，使饲料硒水平分别为０ ．０３ 、０ ．２０ 和０ ．５０ ｍ ｇ／ｋｇ 。训练方式为游泳，每天１ 小时，每周５ 天，持续１０ 周。最后一次训练后７２ 小时宰杀，安静对照小鼠同时宰杀。结果： （１） 补硒状态下长期训练使肝硒、 Ｇ Ｓ Ｈ Ｐｘ 活性显著增加，但这种训练调节在缺硒状态没有发生；低硒状态下长期训练损害了机体抗氧化功能，使 Ｇ Ｓ Ｈ Ｐｘ／ Ｍ Ｄ Ａ 比值显著下降， Ｍ Ｄ Ａ 浓度显著升高。（２） 长期训练使补硒的小鼠脾淋巴细胞 Ｓ Ｉ（ Ｌ Ｐ Ｓ） 和 Ｓ Ｉ（ Ｃｏｎ Ａ） 显著升高，使低硒的小鼠外周血 Ａ Ｎ Ａ Ｅ＋ ％ 显著下降。补硒使训练组小鼠 Ｓ Ｉ（ Ｌ Ｐ Ｓ） 和 Ａ Ｎ Ａ Ｅ＋ ％ 显著升高，而低硒使训练组小鼠 Ｓ Ｉ（ Ｃｏｎ Ａ） 显著下降。（３） 安静对照状态下，硒与机体抗氧化和免疫功能的关系都不明显，而长期训练的小鼠抗氧化功能与免疫功能之间以及硒与二者之间都有显著性相关关系。结论： 补硒有益于运动机?

Objective: To explore the influence of Se status on immune and antioxidative functions of the chronically training mice. Method:C57bl/6J male mice aged four weeks were used and randomly divided into 3 groups: Selenium deficiency (SeD), Selenium normal (SeN) and Selenium supplemented (SeS). Each group was further divided into 2 subgroups: training and resting. Se D mice were fed with a“Se deficient” diet (0.03 mg Se/kg diet). Se N and Se S mice were fed with the same diet supplemented with sodium selenate to reach 0.2 and 0.5 mg Se/kg diet respectively. The exercise applied was forced swimming for 1 hour per day. The duration of the exercise was 5 days per week for 10 weeks. The trained mice were killed 72hr after the last training, and all the resting mice were killed at the same time. Results: (1) Chronically training significantly increased the GSH Px activity and Se content in liver. However this training adaptation did not occur while Se was deficient. Chronically training weakened the antioxidative function under Se deficiency, resulting in the significant decline of GSH Px/MDA ratio and the significant uprise of MDA content.(2) Chronically training significantly enhanced SI(LPS) and SI(ConA) of splenic lymphocyte in Se S mice and significantly decreased the peripheral blood ANAE +% in Se D mice. Se supplementation enhanced SI(LPS) and ANAE +% in training mice, while Se deficiency reduced SI(ConA) in training mice.(3) In non training mice, the correlations among Se antioxidative and immune functions were not significant, while in training mice the correlations were significant. Conclusion: Se supplementation seems to be good to training in terms of enhancing the immune function. The effect of Se on antioxidative function is probably the main contribu tor in its effect on immune function.