尼莫通和川芎嗪对脑缺血-再灌注时c-fos和bcl-2蛋白表达的影响

Effects of Nimotop and Ligustrazini on the protein expression of c-fos and bcl-2 after focal cerebral ischemia followed by reperfusion

目的：为临床应用尼莫通和川芎嗪治疗缺血性脑血管病提供充分的理论依据。方法：对实验大鼠于脑缺血前应用尼莫通和川芎嗪进行药物干预，然后采用免疫组化法检测脑缺血 再灌注不同时间内ｃ ｆｏｓ 及ｂｃｌ ２蛋白表达的变化。结果：①与未用药组相比，尼莫通组和川芎嗪组大鼠脑缺血后的梗死体积显著减小，但两用药组之间相比无显著性差异；②尼莫通组和川芎嗪组脑缺血 再灌注时皮质和基底节区ｃ ｆｏｓ的表达明显下降；而ｂｃｌ ２ 的表达明显增高。

Objective:To provide theoretical basis for clinical application of Nimotop (NM) and Ligustrazini(LZ) in the treatment of ischemic cerebrovascular diseases.Methods:Rats were pretreated with NM( 500 μg/kg ) or LZ(50 mg/kg) 30 min prior to middle cerebral artery occlusion,and changes in protein expression of cfos and bcl2 in rats after ischemiareperfusion were observed by immunohistochemical method.Results:The infarct volume in NM and LZ groups was markedly less than that in the corresponding regions in the control group.In NM and LZ groups,the protein expression of cfos significantly decreased after ischemiareperfusion both in the ischemic cortex and in the ischemic basal ganglia,but the protein expression of bcl2 significantly increased after ischemiareperfusion both in the ischemic cortex and in the ischemic basal ganglia.Conclusions:NM and LZ have the neuroprotective effect against ischemia.