犬脑室内出血动物模型脑细胞超微结构的研究

Ultrastructure study of cerebral cells in intraventricular hemorrhage in dog models

目的：研究脑室内出血后脑细胞超微结构的病理变化及其临床意义。方法：对１８ 只杂种家犬采用脑室内注血法建立脑室内出血动物模型，电镜观察其病理改变。结果：大脑、小脑及脑干神经细胞、胶质细胞及神经纤维细胞内线粒体肿胀及染色质边集、内质网肿胀是其主要病理变化。结论：脑室内注血法是建立犬脑室内出血模型的最简单、最可靠的方法。脑室内出血后脑细胞受损的超微病理改变主要是线粒体肿胀及染色质边集；线粒体肿胀导致ＡＴＰ产生受抑，脑细胞能量不足，从而表现为临床神经功能障碍；染色质边集导致脑细胞死亡。

Objective:To study the ultrastructural pathological changes and their clinical significance.Methods:Experimental intraventricular hemorrhage were produced in 18 mongrel dogs by injection blood into the ventricles,and ultrastructural pathological changes were observed by electron microscopy.Results:Mitochondria swelling,endoplasmic reticulum swelling and chromatin margination were the main pathological manifestations in neurons,neurogliocytes and nerve fiber cells in the cerebrum,cerebellum and brain stem.Conclusions:Canine models of intraventricular hemorrhage by injecting blood into the ventricles appear to be a simple and reliable method.Mitochondria swelling and chromatin margination were main ultrastructural changes of neurocyte damage.Mitochondria swelling would inhibit ATP production,and as a result,the cerebral cells were short of energy,which manifested clinically as neurofunction disturbance.Death of cerebral cells caused by chromatin margination might lead to irreversible nerve cells damage.