摘要
为研究硫丹是否具有雌激素活性，以摘除卵巢的 Ｎ Ｉ Ｈ 雌性小鼠为对象，用多终点体内试验法，观察子宫体重比、子宫积液、动情周期转换、子宫上皮细胞高度及子宫基质细胞层厚度五项指标。结果显示：硫丹组（０ ．５ｍｇ／ｋｇ／ｄ ，１ ．５ｍｇ／ｋｇ／ｄ 和３ ．０ｍｇ／ｋｇ／ｄ） 的五项指标值与阴性对照组相比无显著性差异，且三个组之间无明显的剂量—反应关系。以上结果提示在本实验条件下，硫丹不具有雌激素活性。
We combined five endpoints into a single 5 day in vivo screening assay to identify estrogenicity of endosulfan. The endpoints evaluated as markers of esrogenicity included the ratio of uterine weight/body weight, uterine fluid imbibition, vaginal cornification incidence, uterine stroma cell proliferation and uterine epithelial cell height. The endosulfan were administered daily p.o. for 5 days to sexually mature ovariectomized female NIH mice. Compared with the five endpoints of the three dosage groups of edosulfan (0.5mg/kg/d,1.5mg/kg/d and 3.0 mg/kg/d) showed no significant difference and no significant dose response. Thus, endosulfan has no estrogenicity under the experimental condition.
出处
《癌变．畸变．突变》
CAS
CSCD
1999年第5期252-254,共3页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金
关键词
硫丹
雌激素活性
多终点测定
Endosulfan
Estrogenicity
Multiple endpoints
