发光细菌暗变种对化合物的致突变效应的检测

THE DETECTION OF PHOTOBACTERIUM PHOSPHOREUM DARK MUTANT TO CHEMICALS' MUTAGENIC EFFECT

从明亮发光杆菌 Ｔ３ 小种（ Ｐｈｏｔｏｂａｃｔｅｒｉｕｍ Ｐｈｏｓｐｈｏｒｅｕｍ Ｔ３ Ｓｐ） 分离到的自发暗变种（ｋ 变种） Ｔ９１７１ 菌株检测十种化合物，其中六种化合物呈致突变阳性反应，二种化合物呈可凝阳性，二种化合物呈阴性反应，该方法比较传统的 Ａｍｅｓ 试验检测化合物致突变效应灵敏度高，可望成为一种简易灵敏的测试环境致突变物的短期生物学试验方法。

The positive mutagenic response to six chemicals and doubtable positive mutagenic response to two chemicals and negative mutagenic response to two chemicals were demonstrated by a spontaneous dark mutant (k mutant), Strain T\-\{9171\}, isolated from photobacterium phosphoreum T\-3 Spp. Mutagenic mutant has higher sensitivity to genotoxic chemicals as compared with conventional Ames test. It is possible to become a simple sensitive short term bioloical test method for the detection to mutagenic compounds in environment.