多重PCR对乳腺癌中MTS_1基因外显子2缺失的研究

DELETION OF EXON 2 OF MTS\-1 GENE IN BREAST CARCINOMAS BY MULTIPLEX-PCR

为探索 Ｍ Ｔ Ｓ１ 基因变异在乳腺癌的发生及恶性进程中的作用，作者应用多重 Ｐ Ｃ Ｒ 技术对４７ 例原发性乳腺癌组织进行了检测。结果显示１７ 例纯合性缺失，变异率为３６２ ％ ，其中淋巴结转移组的变异率６０ ％ （９／１５） 显著高于未转移组２５ ％ （８／３２ ， Ｐ＜ ００５） ，浸润性导管癌变异率显著高于其它类型癌。本研究结果提示， Ｍ Ｔ Ｓ１ 基因在乳腺癌组织中有变异存在，其变异的频率与肿瘤的恶性程度及组织分型密切相关。

This study sought to address the relationship of breast carcinoma with deletion of MTS\-1 gene. 17 of 47 cases showed MTS\-1 gene was deleted by multiplex-PCR. The rate of deletion of MTS\-1 gene was 36.2%. the rate of deletion in metastatic breast carcinoma of lymph node was 60%(9/15), that in nonemetastatic breast carcinoma of lymph node was 25%(8/32). There were significant differences in the rate of deletion of MTS\-1 gene between them, and between the rate of infiltrating type of breast carcinoma and the others. These suggest that deletion of MTS\-1 gene was associated with progression and type of breast carcinoma.