大鼠脊髓M受体对α_2受体抗伤害效应的影响

The influence of muscarinic receptor on antinociceptive effects of α_2 adrenergic receptor at spinal level in rats

目的 探讨 α2 受体与 M受体在大鼠脊髓抗伤害效应机制上的相互关系。方法 SD大鼠蛛网膜下腔埋入导管并留置。经导管分别将生理盐水、可乐定、新斯的明、可乐定 +新斯的明、卡巴可、可乐定 +卡巴可注入蛛网膜下腔。以热水甩尾反应阈值为观察指标 ,观察注药前后的抗伤害效应效果。结果 可乐定、新斯的明、卡巴可均可产生时效相关的抗伤害效应 ;可乐定与上述药物合用的抗伤害效应强于单一应用。结论 可乐定、新斯的明、卡巴可大鼠蛛网膜下腔内应用均可产生抗伤害效应 ;脊髓内 α2 受体与

Objective To investigate the antinociceptive mechanism of interaction between α 2 adrenergic receptor and muscarinic receptor at spinal level.Methods Intrathecal catheter was implanted in SD rats The following drugs were administered into subarachnoid through the inserted intrathecal catheter:0.9% NaCl,clonidine,neostigmine,carbachol,clonidine combined with neostigmine,clonidine combined with carbachol.The tail flick threshold by warm water was observed as indicator of antinociceptive effect before and after the administrations.Results Antinociceptive effects were observed following intrathecal clonidine,neostigmine and carbachol administrations and more stronger antinociceptive effects were found after combined administrations than that of alone.Conclusion Intrathecal clonidine,neostigmine and carbachol can produce antinociceptive effect and synergistic antinociceptive effects exis between α 2 adrenergic receptor and muscarinic receptor at spinal level.