联合应用维生素E和维生素C对睡眠剥夺后大鼠心肌氧化应激指标的影响

The effect of vitamin E and vitamin C combination on indicators of oxidative stress in myocardium from sleep deprivation rat model

目的观察联合应用维生素E(VE)和维生素C(VC)对睡眠剥夺后大鼠心肌损伤的保护作用,探讨其作用机制。方法将大鼠随机分8组,每组10只。采用改良多平台睡眠剥夺法(MMPM)建立睡眠剥夺模型,联合VE、VC灌胃给药(VE 500 mg/kg;VC 1.5 g/kg)。观察联合VE、VC对睡眠剥夺后心肌组织丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性的影响。结果与大平台组比较,睡眠剥夺后心肌细胞中MDA的含量明显增加,SOD活性有明显下降趋势;与睡眠剥夺组比较,联合VE、VC组心肌细胞中SOD活性显著提高,细胞中MDA的含量明显降低。提示联合使用VE、VC干预后,可明显降低睡眠剥夺后心肌组织氧化应激反应。结论 联合使用VE、VC可明显降低睡眠剥夺后大鼠心肌氧化应激损伤。

Objective To study the effect of vitamin E and vitamin C combination in protecting impairment of myocardium in rats after sleep deprivation and its mechanism.Methods Experimental rats were randomly divided into 8 groups with 10 in each group.Sleep deprivation rat model was established by ＂Multi-Platform Method＂（MMPM）.Combination of vitamin E and vitamin C（VE 500 mg/kg;VC 1.5 g/kg） was administrated through gastric pouring.The concentrations of superoxide dismutase（SOD） and malondialdehyde（MDA） in myocardical tissue of rats after sleep deprivation were evaluated.Results In comparison with TC group,the result of oxidative stress in myocardium of rats was changed following the increased duration of sleep deprivation,and the concentration of MDA was significantly increased,while the activity of SOD showed a decreasing tendency.The higher activity of SOD and lower concentration of MDA were found after exposure to vitamin E and vitamin C.These results suggested that change of oxidative stress in myocardial tissue after sleep deprivation was significantly attenuated by treatment with vitamin E and vitamin C.Conclusion Vitamin E and vitamin C can decrease the oxidative stress of myocardium in rats after sleep deprivation.