益肾乌发口服液对小鼠急性毒性实验研究

Experimental Study on Mice's Acute Toxicity of Yi Shen Wu Fa Oral Liquid

目的探讨益肾乌发口服液对小鼠急性毒性的影响。方法采用经典的急性毒性试验方法,进行益肾乌发口服液对小鼠的急性毒性研究。结果按生药量计算益肾乌发口服液对小鼠的最大给药量(MLD)为47.5g.kg-1.d-1。相当于人日用剂量的280.0倍;给药后各小鼠急毒主要表现为安静、怠动、腹泻、毛色不华等毒性症状,给药后6天体重增长缓慢,与空白对照组有明显差异,给药后8天逐渐恢复,余未见明显异常,末次给药后2h血ALT、AST水平明显增高,肝体比值增加。结论益肾乌发口服液小鼠急毒虽未做出LD50和MTD,且无一死亡,但药后有肝功能损伤、肝体比值增加,提示在超大剂量灌胃后具有一定的肝毒性,与临床报道相一致。

Objective To discuss the effect of Yi Shen Wu Fa oral liquid on mice＇s acute toxicity.Methods The classical method of acute toxicity were used to study on mice＇s caused by Yi Shen Wu Fa oral liquid.Results Caculated by Yi Shen Wu Fa oral liquid,the MLD is 47.5g·kg-1·d-1,which is equal to 280.0 times of 70kg people＇s daily dried medicinal herb expenses.The main acute toxicity symptoms of mouse are negligent action,diarrhea and hypokinesia.Compared with the control group,The mice＇s body-weight gainings were slow after 6 day＇s administration,which gradually normal after 8 day＇s administration and there were no other obvious abnormalities.The serum concentration of ALT and AST,the liver-body ratio all increased after 2 hours＇ administration.Conclusion The mice＇s acute toxicity of Yi shen wu fa oral liquid could induce the liver injury although could not make the LD50 and MTD,and the mice did not appear dead.It demonstrated that the drug gavages certain dose to mice could cause obvious hepa-injury,which in accordance with the clinical ADR report.