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转基因小鼠中应用RNAi的初步探讨 被引量：3

Application of RNAi in Trangenic Mice
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摘要 RNA干扰(RNAinterference,RNAi)是通过双链RNA介导,特异性地降解相应序列的mRNA,从而导致转录后水平的基因沉默。近年来,利用小分子RNA(small interfering RNA,si RNA)介导的RNAi技术已越来越多的应用于哺乳动物细胞中。同时,在活体中利用RNAi研究多种生物基因功能也成为一种有效的手段。文章旨在介绍在转基因小鼠中RNAi的应用。 RNA interference degrades mRNA specifically and potently though the mediation of corresponding double-stranded RNA and leads to post-transcriptional gene silencing.Recently,small interfering RNA duplexes（siRNA） is used in mammalian cells to silence gene expression.At the same time,RNAi is a powerful tool for studying gene function in vivo.This review provides an overview of transgenic RNA interference in mice.
作者 陈志宝 王东旭 万家余 潘朋朋 李莎 高宏伟
机构地区 黑龙江八一农垦大学生命科学技术学院 中国人民解放军军事医学科学院军事兽医研究所
出处 《中国畜牧兽医》 CAS 北大核心 2011年第5期74-77,共4页 China Animal Husbandry & Veterinary Medicine
基金 国家自然科学基金资助项目(30972197 31072148)
关键词 RNA干扰 RNA双链 转基因小鼠 RNA interference double-stranded RNA transgenic mice
分类号 Q3 [生物学—遗传学]
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参考文献15

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同被引文献74

  • 1边永民.欧盟转基因生物安全法评析[J].河北法学,2007,25(5):157-163. 被引量：19
  • 2陈超,高洪,赵宝洪,屈长林.原位PCR技术及其在病理学中的应用[J].动物医学进展,2007,28(5):95-97. 被引量：8
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引证文献3

中国畜牧兽医
2011年 第5期

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