HPLC测定四味土木香散中土木香内酯和异土木香内酯的含量

Determination of alantolactone,helenin and isoalantolactone in Siwei Tmuxiang powder by HPLC Alisha FU Minghai BAO Baoquan

目的:采用HPLC测定四味土木香散中土木香内酯和异土木香内酯的含量。方法:色谱柱为YMC-Pack ODS-A(250×4.6;5min),流动相为甲醇-乙腈-0.1%磷酸溶液(10:6:9,氨水调pH7),流速为1mL.main-1,检测波长为220nm。结果:土木香内酯进样量2.51～75.3μg、异土木香内酯进样量2.50～75.1μg时与峰面积呈良好的线性;平均回收率分别为:99.86%(RSD=0.32%)和100.34%(RSD=1.12%)。结论:本方法简便、快捷、准确,适用于四味土木香散中土木香内酯和异土木香内酯的质量控制。

objectve Toestablishan RP - HPLCmethodforthedeterminationofalantolactone, heleninandisoalantolactonein Siwei Tmuxiangpowder, methodsSeperationwasachievedonaYMC-PackODS-A（250×46mm;5μm）cohmnanddetectedat220nm.Themobilephaswasthemixtureofmethanol、acetonitrileand 0.1%H3PO4（10.69）at flowrateof 1mL.min-1.REsults The linearrangewas251-753μg foralantolactoneheleninand250-75.1μg for isoalantolactone.Themethodrecoverywas99.86% （RSD=.0.32%）and 100.34%（RSD= 1.12%）conclusionThemethodissimple,quick,accurate ,andissuitableforthedetermination ofalantolactoneheleninandisoalantolactoneinSiweiTmuxiangpowder.