响应面法优化超声提取熟地黄中5-羟甲基糠醛的工艺

Optimization of Ultrasonic Extraction of 5-Hydroxymethylfurfural from Steamed Rehmanniae Radix by Response Surface Methodology

采用响应面法优化了超声提取熟地黄中5-羟甲基糠醛的工艺。选取提取时间、溶剂浓度和温度3个因素为随机因子,在单因素的基础上进行3因素3水平Box-Behnken中心组合试验设计,以5-羟甲基糠醛得率为响应值进行响应面分析。优化后的超声提取熟地黄中5-羟甲基糠醛的条件为:在提取时间61.6min,溶剂体积分数90.5%,提取温度为53.9℃,5-羟甲基糠醛得率理论值为0.3403%,验证值为0.3389%,两者间的相对误差为0.4%,验证值与理论值相吻合,说明该优化方法可行。

Ultrasonic extraction of 5-hydroxymethylfurfural（5-HMF） from steamed Rehmanniae Radix was optimized by response surface methodology.Three factors three levers Box-Behnken design was employed to optimize the extraction process including extraction time,solvent concentration and extraction temperature as the three random factors.The extraction conditions after optimization were as follows：extraction time of 61.6min,methanol concentration of 90.5% and extraction temperature of 53.9℃.The relative error between the predictive yield（0.3403%） of 5-HMF under optimized conditions and results of the verification experiments（0.3389%） was only 0.4%,which indicated that the optimized method was feasible.