摘要
采用水蒸汽蒸馏法(HD)及顶空固相微萃取法(HS-SPME)提取青蒿花的挥发性成分,GC-MS法分析其化学组成。已鉴定化合物占挥发性成分的95.01%(HD)和89.55%(HS-SPME)。其中1,8-桉叶素(21.32%,20.96%),樟脑(24.34%,19.18%),莰烯(3.04%,5.26%),侧柏烯(3.72%,4.08%),α-萜品醇(2.30%,1.16%),2-丙烯基-4a,8-二甲基-1,2,3,4,4a,5,6,7-八氢萘(3.40%,4.69%),β-法尼烯(4.08%,1.62%)分别为HD及HS-SPME主要挥发成分;它们在挥发性成分中的相对含量分别为62.20%(HD)和56.95%(HS-SPME)。结果表明2种提取法所得挥发性成分中的主要成分没有明显差别。HS-SPME与HD法相比,具有样品用量少、提取时间短、能耗低、环境友好的特点;且提取物不需进一步纯化,溶剂转换以及过滤等烦琐操作即可直接进行分析,是富集青蒿花挥发性成分的理想方法。
The chemical components of essential oils that were extracted from Artemisia annua flowers by different methods were compared.The volatile extracts were obtained by traditional hydro-distillation(HD) and headspace solid-phase microextraction(HS-SPME) methods from Artemisia annua flowers,and the chemical components were analyzed by gas chromatograph-mass spectrometry(GC-MS) system.The identified components were 95.01%(HD) and 89.55%(HS-SPME) of the volatile chemical composition of flowers,respectively.The main constituents were 1,8-cineole(21.32%,20.96%),camphor(24.34%,19.18%),camphene(3.04%,5.26%),sabinene(3.72%,4.08%),α-terpineol(2.30%,1.16%),β-farnesene(4.08%,1.62%) and 2-isopropenyl-4a,8-dimethyl-1,2,3,4,4a,5,6,7-octahydronaphthalene(3.40%,4.69%),which were 62.20% and 56.95% of all volatile composition by HD and HS-SPME,respectively.There was no significant difference of the main chemical components,and their contents of the volatile composition were extracted by HD and HS-SPME.The HS-SPME method offers important advantages over traditional alternatives,namely:less samples,shorter isolation time,lower energy consumption,cleaner features.Furthermore,the extractions of volatile composition from HS-SPME could be analysed directly without any clean-up,solvent exchange or centrifugation steps.HS-SPME is a good method for the extraction of volatile composition from Artemisia annua flowers.
出处
《光谱实验室》
CAS
CSCD
北大核心
2011年第3期1276-1279,共4页
Chinese Journal of Spectroscopy Laboratory
基金
重庆大学研究生创新基金(200706B1B0150235)