真武汤的HPLC指纹图谱研究

Study on HPLC fingerprint chromatography of Zhenwu decoction

目的:应用HPLC法建立真武汤的指纹图谱。方法:以Phenomenex Luna C185μm反相色谱柱为分析柱,0.025%甲酸水溶液(用氨水调pH=3.5)-乙腈为流动相,梯度洗脱,流速1 mL.min-1,检测波长242 nm,柱温30℃,进样量20μL。结果:本色谱条件下真武汤各成分得到较好的分离,经方法学验证,该方法具良好精密度、重复性和稳定性。采用中药色谱指纹图谱相似度评价系统软件(2004 A版)进行指纹图谱分析,10批真武汤样品相似度均在0.90以上。结论:本方法建立的真武汤指纹图谱,用于真武汤的质量控制。

Objective：To establish HPLC fingerprint of analyzing Zhenwu decoction.Methods：The separation was applied with a Phenomenex Luna C18（4.6 mm×250 mm,5 μm）column by gradient elution with acetonitrile-0.025% formic acid solution（adjusted to pH 3.5 with aqueous ammonia）as the mobile phase.The flow rate was 1 mL·min-1 and the detection wavelength was set at 242 nm.The injection volume was 20 μL and the column temperature was maintained at 30 ℃.Results：Among the obtained fingerprint,the most of the detected peaks were separated effectively.The accuracy,repeatability,and stability of this method were satisfied.The operating standard of Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica（Version 2004 A）was used to calculate.Similarity of 10 batches of Zhenwu decoction samples was higher than 0.90.Conclusion：The method can be well used for the component analysis and the quality control of Zhenwu decoction.