摘要
目的:建立反相高效液相色谱法测定复方鱼腥草片中(R,S)-告依春的含量。方法:采用C18色谱柱,以0.05%磷酸溶液-乙腈(93∶7)为流动相,流速0.8 mL·mL^-1,检测波长245 nm,柱温30℃。结果:(R,S)-告依春在0.028~0.280 mg·mL^-1范围内具有良好的线性关系,r=0.9995;平均回收率为99.7%。结论:该方法可作为复方鱼腥草片的质量控制方法。
Objective:To establish an RP-HPLC method for determination of(R,S)-epigoitrin in compound Yuxingcao tablets.Methods:A C18 column was adopted with 0.05% phosphoric acid-acetonitrile(93∶7)as the mobile phase at a flow rate of 0.8 mL·min^-1,the detection wavelength was 245 nm and the column temperature was 30 ℃.Results:The calibration curve was linear in the range of 0.028-0.280 mg·mL^-1(r=0.9995).The average recovery was 99.7%.Conclusion:The method can be used for determination of(R,S)-epigoitrin in compound Yuxingcao tablets.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2011年第5期900-902,共3页
Chinese Journal of Pharmaceutical Analysis