RP-HPLC法测定罗布麻叶及其中成药中3个黄酮类成分的含量

RP-HPLC determination of three flavones in Apocyni Veneti Folium and its Chinese traditional patent medicine

目的：建立RP-HPLC测定罗布麻叶及其中成药中白麻苷、芦丁、异槲皮苷3个黄酮类成分含量的方法。方法：罗布麻叶和及其中成药经甲醇超声提取后,以HPLC法测定。采用Diamosil C18（250 mm×4.6 mm,5μm）色谱柱,流动相为乙腈-0.1%醋酸水溶液,梯度洗脱,流速1.0 mL·min^-1,检测波长360 nm,柱温为室温。结果：白麻苷、芦丁及异槲皮苷进样量分别在0.4-10μg（r=0.9996）、0.01-0.25μg（r=0.9998）及0.075-1.875μg（r=0.9997）范围内与峰面积呈良好的线性关系。平均回收率（n=5）分别为99.7%,100.8%,99.7%;RSD分别为0.9%,3.2%,2.2%。结论：本方法简单、快速、准确,重复性好,为罗布麻药材及其中成药的质量评价提供了可靠的参考依据。

Objective：To establish an HPLC method for the determination of three flavones（baimaside,rutin,isoquercitrin） in Apocyni Veneti Folium and its Chinese traditional patent medicine.Methods：Apocyni Veneti Folium and its Chinese traditional patent medicine were extracted with methanol by ultrasound extraction;The chromatographic procedure was carried out with Diamosil C18（250 mm×4.6 mm,5 μm） column,gradient elution with the mobile phase of acetonitrile-0.1% acetic acid solution at a flow of 1.0 mL·min-1,the detection wavelength was 360 nm,and the column temperature was room temperature.Results：The linear ranges of baimaside,rutin and isoquercitrin were 0.4-10 μg（r=0.9996）,0.01-0.25 μg（r=0.9998） and 0.075-1.875 μg（r=0.9997）,respectively;The average recoveries（n=5） were 99.7%（RSD=0.9%）,100.8%（RSD=3.2%）and 99.7%（RSD=2.2%）,respectively.Conclusion：The method is simple,rapid,accurate and reproducible,which can offer credible quality control method for Apocyni Veneti Folium and its Chinese traditional patent medicine.