RP-HPLC同时测定党参中党参炔苷与苍术内酯Ⅲ

RP-HPLC simultaneous determination of lobetyolin and atractylenolide Ⅲ in Radix Codonopsis

目的：建立同时测定党参中党参炔苷及苍术内酯Ⅲ含量的HPLC分析方法。方法：采用Symmetry C18（4.6 mm×250 mm,5μm）色谱柱,流动相为乙腈（A）-水（B）,梯度洗脱（0-25 min,10%A→40%A;25-35 min,40%A→100%A）,流速0.8 mL·min^-1,检测波长220 nm,柱温30℃,进样量10μL。结果：党参炔苷与苍术内酯Ⅲ分离良好,进样量分别在0.076-1.52μg（r=0.9997）与0.088-1.76μg（r=0.9998）范围内线性关系良好,平均回收率分别为99.81%（RSD=1.1%）及100.1%（RSD=0.94%）。结论：该方法操作简便、准确,具有良好的重复性,可作为党参中党参炔苷与苍术内酯Ⅲ同时测定的有效方法。

Objective：To develop an RP-HPLC method for simultaneous determination of lobetyolin and atractylenolide Ⅲ in Radix Codonopsis.Methods：Symmetry C18（4.6 mm×250 mm,5 μm）was adopted,the mobile phase was acetonitrile and water with the linear gradient elution（0 min,10% acetonitrile;25 min,40% acetonitrile;35 min,100% acetonitrile）at the flow rate of 0.8 mL·min-1,the UV detector was set at 220 nm,the column temperature was 30 ℃ and the injection volume was 10 μL.Results： The resultant correlation coefficients of lobetyolin and atractylenolide Ⅲ were 0.9997 and 0.9998 with range from 0.076 to 1.52 μg,from 0.088 to 1.76 μg,respectively.The average recoveries of lobetyolin and atractylenolide Ⅲ were 99.81% with RSD of 1.1%,and 100.1% with RSD of 0.94%,respectively.Conclusion：This method can be applied to the analysis of lobetyolin and atractylenolide Ⅲ in Radix Codonopsis.The results indicated this method is simple,rapid and accurate.