HPLC法测定金鸡片中3种生物碱的含量

HPLC determination of three alkaloids in Jinji tablets

目的：建立HPLC法测定金鸡片中盐酸药根碱、盐酸巴马汀和盐酸小檗碱3种生物碱的含量。方法：选用WCX弱阳离子交换固相萃取小柱净化,采用Hvydro-RP 80A C18（250 mm×4.6 mm,5μm）色谱柱,以乙腈-0.05 mol·L^-1磷酸二氢钠溶液（磷酸调pH 2.8）（27∶73）为流动相,流速1.0 mL·min^-1,检测波长265 nm,柱温35℃。结果：盐酸药根碱、盐酸巴马汀、盐酸小檗碱进样量分别在4.94-49.44 ng（r=1.000）、4.36-43.60 ng（r=0.9999）和3.99-39.94 ng（r=0.9999）范围内线性关系良好;回收率（n=9）分别为98.32%,99.53%,98.35%。结论：该方法操作简便,准确性、重复性好,可用于金鸡片中盐酸药根碱、盐酸巴马汀和盐酸小檗碱的含量测定。

Objective：To establish an HPLC method for the determination of jatrorrhizine hydrochloride,palmatine hydrochloride and berberine hydrochloride in Jinji tablets.Methods：WCX weak cation SPE column was used to clean.The Hvydro-RP 80A C18 column（250 mm×4.6 mm,5 μm）was used at 35 ℃ with acetonitrile-0.05 mol·L^-1 sodium dihydrogen phosphate（pH 2.8）（27∶73）as the mobile phase,1.0 mL·min^-1 as the flow rate,265 nm as the detective wavelength.Results：The linear ranges were 4.94-49.44 ng（r=1.000）for jatrorrhizine hydrochloride,4.36-43.60 ng（r=0.9999）for palmatine hydrochloride and 3.99-39.94 ng（r=0.9999）for berberine hydrochloride.The average recoveries（n=9）were 98.32%,99.53% and 98.35%,respectively.Conclusion：This method is simple,accurate and repeatable.It can be used for the determination of jatrorrhizine hydrochloride,palmatine hydrochloride and berberine hydrochloride in Jinji tablets.