流动注射化学发光法测定姜黄素

Determination of curcumin by flow injection chemiluminescence method

目的：以Ce（Ⅳ）作为氧化剂,建立化学发光法测定中药成分姜黄素。方法：用Ce（Ⅳ）直接氧化姜黄素能产生弱发光,而罗丹明B（RhB）能大大增强此弱发光,由此建立了测定姜黄素的FIA-CL分析法。结果：测定姜黄素的线性范围为5.0×10^-9-1.0×10^-7mol·L^-1,其检出限为3.0×10^-9mol·L^-1;对于1.0×10^-8mol·L^-1姜黄素进行11次测定的RSD为2.9%。结论：本方法简便、快捷且具有较高的灵敏度,可用于中药姜黄中姜黄素的分析。

Objective：To establish a new method of determination of curcumin in Ce（Ⅳ）oxidation chemiluminescence（CL）systems.Methods：It was found that mixing of Ce（Ⅳ）and curcumin in the acidic condition produced weak chemiluminescence,which could be greatly sensitized by rhodamine B（RhB）.Based on the above discovery,a FIA-CL method for the determination of curcumin was established.Results： The linear range of this method was 5.0×10^-9–1.0×10^-7mol·L^-1 for curcumin with the detection limit of 3.0×10^-9mol·L^-1and relative standard deviation（n=11,C=1.0×10^-8mol·L^-1）of 2.9 %.Conclusion：This method is simple,quick and reliable.It has been applied to the determination of curcumin in Curcuma Longa L.with satisfactory results.