孵化过程中受精蛋与非受精蛋抗肠炎沙门菌性的比较

Comparison of Anti-Salmonella Enteritidis Properties between Inoculated Fertile Eggs and Infertile Eggs During Incubation

为探明受精蛋在孵化过程中的抑菌机制,本研究对不同孵化阶段,受精蛋与非受精蛋抗肠炎沙门菌性进行了比较研究。利用1×105 cfu/mLSalmonella Enteritidis(SE)菌悬液浸泡接种受精蛋与非受精蛋,测定蛋壳与壳膜、蛋清、蛋黄在不同孵化时间SE的侵染情况。结果表明:孵化第4天,受精蛋蛋壳与壳膜、蛋清比非受精蛋抗肠炎沙门菌活性强(P<0.05),两种鸡蛋蛋黄样本均未受到侵染;第8、12天,两种鸡蛋3个部位肠炎沙门菌的侵染情况没有显著性差异(P>0.05);第17天,受精蛋蛋壳与壳膜、蛋黄抗肠炎沙门菌活性显著高于非受精蛋(P<0.05),此时受精蛋鸡胚的漂洗物肠炎沙门菌阳性率为13.78%,非受精蛋蛋清肠炎沙门菌阳性率为45.16%。

In order to verify the anti-bacterial mechanism of fertile eggs during incubation,the fertile and infertile eggs were used in this experiment to identify the resistance against Salmonella Enteritidis （SE） during different stages of incubation. They were put into inoculation in a SE suspension of approximately l xl05 CFU/mL. Egg shell and membrane, albumen,yolk were sampled respectively. The results showed that it was significantly that fewer of the fertile eggs＇ shell and membrane,albumen samples were contaminated on days 4 （P〈0.05）,the yolk samples of the two kinds of eggs were not comtaminated. There were no significant differences between fertile an infertile eggs in all the sample tested on 8 days and 12 days after incubation （P〉0.05）. However,on 17 days after incubation,it was significantly that fewer of the fertile eggs＇ albumen and yolk samples were contaminated （P〈0.05） ,moreover,13.78% of the chick rinses samples from fertile eggs were positive,45.16% of the albumen samples from infertile eggs were positive.