摘要
辅酶Q是存在于线粒体内膜上的脂溶性电子传递体,它是呼吸链的重要组成部分.辅酶Q在不同生物体内其侧链异戊二烯单位的数目不同,目前对黑腹果蝇(Drosophila melanogaster)中辅酶Q的存在形式和含量还不清楚.利用高效液相色谱法分析鉴定了模式生物黑腹果蝇中辅酶Q的种类及含量.实验结果表明:黑腹果蝇中辅酶Q9与Q10并存,辅酶Q9的含量约为Q10的2.5倍.辅酶Q含量具性别差异,其中辅酶Q9在雌性黑腹果蝇中含量较雄性约高30%,而辅酶Q10在雌性果蝇中则比雄性果蝇高约33%;辅酶Q9与Q10含量在辅酶Q合成酶coq2的突变体中明显降低并且coq2突变体的寿命较野生型略短.这些实验结果将为利用黑腹果蝇模型研究辅酶Q的功能提供重要基础.
Coenzyme Q is an essential component of the mitochondrial respiratory chain as a lipid-soluble electron transporter located in the inner membrane of mitochondria.The number of isoprenoid subunits in the coenzyme Q side chain varies in different species.The content of coenzyme Q in Drosophila has not been reported yet.In the present report,we analyzed the content of coenzyme Q in adult Drosophila by HPLC.We provided evidence that Drosophila contains both CoQ9 and CoQ10.The amount of CoQ9 is about 2.5 folds than that of CoQ10.We further showed the content of CoQ9 and CoQ10 in females are respectively about 30%,33% higher than that in males.In addition,both concentrations of CoQ9 and CoQ10 are significantly reduced in the mutant of CoQ synthase gene coq2 which appeared with shortened life-span.These results provided a good basis to further investigate in vivo biological functions of CoQ in Drosophila.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2011年第3期607-611,共5页
Journal of Xiamen University：Natural Science
基金
国际科技合作项目(2009DFA32760)
