旱地小麦根际细菌中产生1-氨基环丙烷-1-羧酸(ACC)脱氨酶菌株的分离和鉴定

Isolation and identification of wheat rhizosphere bacteria producing 1-aminocyclopropane-1-carboxylate(ACC) deaminase from drought soil

采用富集定向筛选法,从旱地小麦的根际土壤中分离到2株产生1-氨基环丙烷-1-羧酸(ACC)脱氨酶的菌株AS和CS。经测定菌株AS和CS的ACC脱氨酶的比活力分别为0.018 6 U/mg和0.016 7 U/mg蛋白。根据培养特征观察和生理生化指标测定结果,结合16S rDNA碱基序列测定和系统发育同源性分析,确定菌株AS和菌株CS分别属于霍氏肠杆菌(Enterobacter hormaechei)和变形斑沙雷氏菌(Serratia proteamaculans)。

Two bacteria strains marked AS and CS,producing 1-aminocyclopropane-1-carboxylate（ACC） deaminase,were isolated from wheat rhizosphere of drought soil.The activity of ACC deami-nase from AS and CS strains was 0.018 6 U/mg and 0.016 7 U/mg protein,respectively.Based on the cultural morphological features as well as physiological and biochemical parameters in combination with 16S rDNA sequence analysis and sequence homology analysis,the strain AS and CS were identi-fied as Enterobacter hormaechei and Serratia proteamaculans,respectively.