iNOS在肺癌中的表达及其与肿瘤病理、临床分期的关系

Study of the Expression of iNOS of Lung Cancer with Different Pathological Types of Clinical Stages

采用逆转录聚合酶链反应（ＲＴＰＣＲ） 技术检测４０例不同肿瘤病理、临床分期的肺癌组织中诱生型一氧化氮合酶 （ｉＮＯＳ） ｍ ＲＮＡ的表达情况。结果表明， 肺癌组ｉＮＯＳｍ ＲＮＡ阳性表达率为７２５０％ ， 较对照组（１１７６％ ） 明显增高（Ｐ＜ ００１）。ｉＮＯＳ阳性表达与病理学类型无关， 与细胞分化程度呈负相关。早期肺癌ｉＮＯＳ阳性率（８７５０％ ） 显著高于晚期肺癌的阳性率（５０００％ ）。提示肺癌组织中存在ｉＮＯＳ， 它可能通过合成一氧化氮（ＮＯ） 在分子水平参与了肺癌的发生、发展。

We detected the expression of inducible nitric oxide synthase(iNOS)mRNA by reverse transcription polymerase chain reaction(RT-PCR)in 40 cases of lung cancer patients The results showed that the target gene of iNOS were detected in 29 of 40 tumor tissues(72 50%),while in 2 of 17 normal tissues(11 76%),P<0 01。No significant difference was found among the expression of iNOS mRNA in different pathological types,but the poorly differentiated tumor cell showed a higher iNOS expression than moderate differentiated tumor cell(P<0 05) The early clinical stages(Ⅰ～Ⅱ)of lung cancer have an increased frequency of iNOS expression compaired with the advanced stages(Ⅲ～Ⅳ)(P<0 05) It suggests that iNOS gene expression occurs in human lung cancer,it might involve in the development of lung cancer in gene level by generating NO