大棚翠冠梨果实糖积累及蔗糖代谢相关酶活性的研究

Study on enzyme activities involved in sugar accumulation and sucrose metabolization in Cuiguan pear under plastic tunnel culture

以大棚翠冠梨为试材(露地栽培作对照),研究了果实生长发育过程中糖积累以及与蔗糖代谢相关酶活性的季节变化,并对果实中糖积累与酶活性关系进行了分析。结果表明,随着果实生长发育,大棚和露地翠冠梨中山梨糖醇、果糖、葡萄糖和蔗糖持续积累。与露地栽培相比,大棚栽培降低了总糖特别是蔗糖的含量。酸性转化酶(AI)和中性转化酶(NI)在果实发育前期活性很强,随着果实发育而迅速下降。蔗糖合成酶分解方向(SSc)活性随果实发育而下降,而合成方向(SSs)活性则随果实发育而逐步增强。蔗糖磷酸合成酶(SPS)活性随果实发育持续上升,在果实发育的中后期急剧升高,与蔗糖迅速积累的时期相一致。大棚栽培显著降低了果实SPS活性,是果实蔗糖含量下降的主要原因。

Enzyme activities involved in sugar accumulation and sucrose metabolization of were studied during the fruit development of Cuiguan pear（Pyrus pyrifolia Nakai.） produced under plastic tunnel culture（PL） and in the open field（OF）.Results showed that the content of sorbitol,glucose,fructose and sucrose increased with the fruit development.Total sugar especially sucrose content of fruit produced under plastic tunnel culture（PL） was lower than that in the open field（OF）.The activities of acid invertase（AI） and neutral invertase（NI） were high at early stage and decreased strongly with fruit development.The sucrose synthase activity in the sucrose cleavage direction（SSc） reduced while that in the sucrose synthesis direction（SSs） increased gradually with fruit development.SPS activity increased slowly during the early stage of fruit growth,but showed a steep rise afterwards when sucrose accumulated sharply.The activities of SPS were significantly lower under plastic tunnel culture（PL） than that in the open field（OF） which leads to the loss of sucrose content under plastic tunnel culture（PL）.