我国葡萄主栽区卷叶病相关病毒种类的检测分析

Study of Grapevine leafroll-associated viruses in China

田间调查200个葡萄品种,结果82个品种表现葡萄卷叶病症状,其中欧亚种群表现葡萄卷叶病症状的品种最多,发病率最高,发病症状最重;欧美杂种发病率和严重度均比欧亚种群低;未发现美洲种群的品种表现葡萄卷叶病症状。从58株表现卷叶病的葡萄上采集休眠枝条进行卷叶病毒ELISA和RT-PCR检测,葡萄卷叶病毒-1,2,3,4,5和7(GLRaV-1,2,3,4,5和7)等6种葡萄卷叶病毒的检出率分别为20.7%,17.2%,62.1%,3.4%,5.2%和15.5%。回收PCR产物进行克隆和序列分析,并将测序结果提交至GeneBank。以葡萄卷叶病毒HSP70基因序列构建系统进化树分析其进化关系,GLRaV-1、3和7,GLRaV-2、4和5分别聚为2大类,其中GLRaV-1和3,GLRaV-4和5在各自的聚类中关系更近。

Two hundred grapevine cultivars were surveyed for the prevalence of grapevine leafroll disease（GLD）.82 cultivars showed GLS symptoms,and the cultivars belong to Vitis vinifera L.had more cultivars,higher morbidity and more serious symptoms than V.vinifera-V.labrusca.No V.labrusca L.was found showing GLD symptoms in the vineyards.Fifty eight samples collected from the vineyards were tested for GLRaVs by ELISA and RT-PCR.The positive rate of GLRaV-1,2,3,4,5 and 7 was 20.7%,17.2%,62.1%,3.4%,5.2% and 15.5% respectively.The recovered fragments were cloned,sequenced and identified.The sequencing results have been submitted to GenBank.In this paper,the evolutionary relationship of GLRaVs was conducted based on HSP70 encoding genes.Neighbor-Joining（NJ）phylogenetic trees was established,and GLRaV-1,3 and 7,GLRaV-2,4 and 5 were classified together in a cluster respectively.Moreover,GLRaV-1 and 3 have closer genetic relation than GLRaV-7.And GLRaV-4 and 5 have closer genetic relation than GLRaV-2.