期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

耐有机溶剂脂肪酶产生菌的筛选和鉴定 被引量：2

The Screening and Identification of a Novel Strain Producing an Organic Solvent-Tolerant Lipase
下载PDF
导出
摘要 从国内各地采取土样分离筛选产耐有机溶剂脂肪酶的新菌株,通过形态和生理生化特征以及系统进化树分析,筛选到的新菌株命名为Pseudomonas aeruginosa CS-2.来自Pseudomonas aeruginosa CS-2的粗脂肪酶液在乙腈中酶活提高,在苯、氯仿、正己烷、石油醚和异辛烷中表现出较高的稳定性. A novel strain which produced an organic solvent-tolerant lipase was isolated from soils in different areas of China.The strain was identified as Pseudomonas aeruginosa CS-2 by morphological,physiological and biochemical features and phylogenetic tree analysis.The crude lipase was activated in the presence of acetonitrile and it exhibited high stability in presence of benzene,chloroform,n-Hexane,petroleum ether and isooctane.
作者 彭仁 林金萍 魏东芝
机构地区 华东理工大学反应器工程国家重点实验室 江西师范大学生命科学学院
出处 《江西师范大学学报（自然科学版）》 CAS 北大核心 2011年第2期197-200,共4页 Journal of Jiangxi Normal University(Natural Science Edition)
基金 国家重点基础研究发展计划(2009CB724703) 江西省科技支撑计划(200800361)资助项目
关键词 铜绿假单胞菌 有机溶剂耐受性 脂肪酶 Pseudomonas aeruginosa organic solvents tolerance lipase
分类号 Q93 [生物学—微生物学]
  • 相关文献

参考文献18

  • 1Serdakowsli A L,Dordick J S.Enzyme activation for organic solvents made easy[J].Trends Biotechnol,2008,26(1):48-54.
  • 2Sardessai Y,Bhosle S.Industrial potential of organic solvent tolerant bacteria[J].Biotechnol Prog,2004,20(3):655-660.
  • 3Inoue A,Horikoshi K.A Pseudomonas thrives in high concentrations in touene[J].Nature,1989,338 (16):264 -266.
  • 4Sardessai Y,Bhosle S.Organic solvents tolerant bacteria in mangrove ecosystem[J].Curr Sci,2002,82(6):62-623.
  • 5Ogino H,Miyamoto K,Ishikawa H.Organic-solvent-tolerant bacterium which secretes organic-solvent-stable lipolytic enzyme[J].Appl Environ Microbiol,1994,60(10):3884-3886.
  • 6Rahaman RNZRA,Baharum S N,Salleh A B.S5 lipase:an organic solvent tolerant enzyme[J].J Microbiol,2006,44(6):583-590.
  • 7Hun C J,Rahaman R N Z A,Salleh A B.A newly isolated organic solvent tolerant Bacillus sphaericus 205y producing organic solvent-stable lipase[J].Biochem Eng J,2003,15(2):147-157.
  • 8Zhao Lili,Xu Jianhe,Zhao Jian,et al.Biochemical properties and potential applications of an organic solvent tolerant lipase isolated from Serratia marcescens ECU1010[J].Process Biochem,2008,43(6):626-633.
  • 9沈萍,陈向东.微生物实验[M].4版.北京:高等教育出版社,2007.
  • 10Weisburg W G,Barns S M,Pellatier D A,et al.16S ribosomal DNA amplification for phylogenetic study[J].J Bacteriol,1991,173(2):697-703.

共引文献10

同被引文献16

  • 1陈源源,牛丹丹,王正祥.一种快速鉴定细菌的方法[J].食品与发酵工业,2007,33(5):29-31. 被引量：8
  • 2Hasan F, Shah A A, Hameed A. Industrial applications of microbial lipases [ J ]. Enzyme and Microbial Technology, 2006,39:235-251.
  • 3Houde A, Kademi A, Leblanc D. Lipases and their indus- trim applications [ J ]. Applied Biochemistry and Biotech- nology,2004,118 : 155-170.
  • 4Treichel H, de Oliveira D, Mazutti M A, et al. A review on microbial lipases production [ J ]. Food and Bioprocess Technology,2010,3 : 182-196.
  • 5Jaeger K E, Liebeton K, Zonta A, et al. Biotechnologieal application of Pseudomonas aeruginosa lipase: efficient ki- netic resolution of amines and alcohols [ J ]. Applied Mi- crobiology and Biotechnology, 1996,46:99-105.
  • 6Jaeger K E, Eggert T. Lipases for bioteehnology [ J ]. Cur- rent Opinion in Biotechnology,2002,13:390-397.
  • 7Li Xiaojun, Zheng Renchao, Wu Zheming, et al. Ther- mophilic esterase from Thermomyces lanuginosus:Molecu- lar cloning,functional expression and biochemical charac- terization [ J]. Protein Expression and Purification,2014, 101:1-7.
  • 8Petersen T N, Bmnak S, yon Heijne G, et al. $ignalP 4. 0 : discriminating signal peptides from transmembrane regions [J]. Nature Methods,2011,8:785-786.
  • 9Wang Xiaoqing, Yu Xiaowei, Xu Yan. Homologous expres- sion, purification and characterization of a novel high-alka- line and thermal stable lipase from Burkholderia cepacia ATCC 25416 [ J]. Enzyme and Microbial Technology,2009,45:94-102.
  • 10Bae W, Chen W, Mulehandani A, et al. Enhanced bioaccu- mulation of heavy metals by bacterial cells displaying syn- thetic phytochelatins [ J ]. Biotechnology and Bioengineer- ing,2000,70:518-524.

引证文献2

二级引证文献15

江西师范大学学报（自然科学版）
2011年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部