女贞子对大鼠成骨细胞增殖与分化的影响

Effects of Aqueous Extract of Fructus Ligustri Lucidi on the Proliferation and Differentiation of Osteoblastic UMR-106 cells

为探讨女贞子Fructus Ligustri Lucidi体外对大鼠成骨样细胞UMR-106增殖与分化的影响,女贞子水提物（LWE）以不同浓度加入细胞培养体系,用Am-Blue细胞增殖与活性检测试剂检测成骨细胞的增殖情况;以检测细胞内碱性磷酸酶的活性为指标考察成骨细胞的分化情况。结果表明,LWE在100μg/mL作用48 h能促进细胞的增殖,作用24~48 h能明显促进细胞的分化。在转染了5×ERE-Luc荧光素酶报告基因质粒的乳腺癌细胞MCF-7中检测到LWE能促进雌激素受体反应元件调控下的荧光素酶的表达;且LWE促UMR-106细胞分化的作用能被雌激素受体拮抗剂ICI18270所抑制,表明女贞子很可能是通过雌激素受体信号途径对成骨细胞的分化起作用的。

To study the effects of Fructus Ligustri Lucidi on the proliferation and differentiation of cultured osteoblasts in vitro,the proliferation and the activity of alkaline phosphatase（ALP） in osteoblastic UMR-106 cells was investigated in the presence of water extract of Fructus Ligustri Lucidi（LWE） with Am-blue method and p-nitrophenyl phosphate disodium salt（PNPP） method respectively.The results showed that LWE promoted the proliferation and differentiation of UMR-106 for 48 h at 100 μg/mL.To study the mechanism of LWE on differentiation,pGL3-5×ERE-Luc reporter plasmid was transfected into breast cancer cell MCF-7,and the effect of LWE on the expression of luciferase was investigated.The results indicated that LWE promoted the estrogen receptor responsive element（ERE） driven expression of luciferase.The promoting effect of LWE on the differentiation of UMR-106 cells could be suppressed by ICI182780,an estrogen receptor antagonist.These results suggested that the mechanism of differentiation promoting effect by LWE may be related to estrogen receptor signaling pathway.