胰蛋白酶水解β-乳球蛋白获得ACE抑制肽的条件优化

Optimizing Hydrolysis Conditions of β-Lactoglobulin with Trypsin to Obtain the ACE Inhibitory Peptide

采用三因素二次通用旋转设计和体外检测法,对胰蛋白酶水解β-乳球蛋白获得ACE抑制肽的条件进行优化。结果表明,底物浓度(X1)、温度(X2)、酶与底物的质量比(X3)对ACE抑制率的影响回归方程为:Y=50.62-2.33X1-1.97X2+5.81 X3-3.36X2X3-6.56X22-1.96X32,胰蛋白酶水解β-乳球蛋白获得ACE抑制肽的最优水解条件为:底物质量浓度为60 g/L,水解温度30℃,酶与底物的质量比为5.5%,水解时间6 h,水解产物对ACE抑制活性最大抑制率为53.86%。

Three-factor quadratic orthogonal rotation design and in vitro assay was adopted to optimize the hydrolysis conditions of β-lactoglobulin with trypsin for obtaining the Angiotensin-I-converting enzyme（ACE） inhibitory peptide.The results showed that the regression equation of the impacts of the substrate concentration（X1）,temperature（X2）,enzyme,and substrate mass ratio（X3） on the ACE inhibition rate was Y = 50.62-2.33X1-1.97X2＋5.81 X3-3.36X2X3-6.56X22-1.96X32.The optimum conditions were as following：when substrate concentration was at 60 g/L,the hydrolysis temperature at 30 ℃,enzyme and substrate mass ratio at 5.5%,the hydrolysis time in 6 h,the hydrolysis product of ACE inhibitory activity was on the maximal inhibition rate of 53.86%.